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Rat GM-CSF Recombinant Protein
- Catalog Number:
- RP1193R
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Rattus norvegicus (Norway rat)
- Rat GM-CSF (Granulocyte-Macrophage Colony-Stimulating Factor) (catalog RP1193R) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~14.5 kDa, 127 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to Norway rat. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study GM-CSF signaling and hematopoiesis (including regulation of granulocyte and macrophage differentiation and activation); typical experimental uses include cell-culture stimulation and differentiation assays, cytokine signaling assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | APTRSPNPVT RPWKHVDAIK EALSLLNDMR ALENEKNEDV DIISNEFSIQ RPTCVQTRLK LYKQGLRGNL TKLNGALTMI ASHYQTNCPP TPETDCEIEV TTFEDFIKNL KGFLFDIPFD CWKPVQK (127) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 14.5 kda |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Rat GM-CSF (Granulocyte-Macrophage Colony-Stimulating Factor, CSF2) is a hematopoietic cytokine belonging to the colony-stimulating factor family, which also includes G-CSF (CSF3) and M-CSF (CSF1) that regulate the development, proliferation, and activation of myeloid immune cells. In rats (Rattus norvegicus), GM-CSF is produced by activated T lymphocytes, macrophages, endothelial cells, fibroblasts, and epithelial cells in response to infection, inflammatory cytokines, or immune stimulation. Rat GM-CSF binds to the GM-CSF receptor (CSF2R) expressed on hematopoietic progenitor cells and mature myeloid cells, activating intracellular signaling pathways such as JAK/STAT, MAPK/ERK, and PI3K-AKT, which promote the proliferation and differentiation of granulocytes, macrophages, and dendritic cells and enhance their phagocytic and antigen-presenting functions. In rat immunity, GM-CSF plays an important role in regulating inflammatory responses and innate immune defense, particularly through activation of macrophages and neutrophils during infection or tissue injury. Rats are widely used as animal models for inflammatory and respiratory diseases, and GM-CSF-regulated myeloid responses have been studied in rat models of acute lung injury, where increased GM-CSF signaling influences macrophage activation and inflammatory cell recruitment in the lung, helping researchers better understand mechanisms of pulmonary inflammation and immune-mediated tissue damage relevant to human disease.
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