Rabbit IL-5 Recombinant Protein

Catalog Number:
RP1231U
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Oryctolagus cuniculus (rabbit)
  • Rabbit IL-5 (Interleukin-5) (catalog RP1231U) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~13.4 kDa, 115 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to rabbit. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study IL-5 signaling and immune responses (including regulation of eosinophil growth, differentiation, and activation); typical experimental uses include cell-culture stimulation and dosing studies, cytokine signaling assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceMATEIRMSTV VKETLTLLST YQSLLIGNET LMIPVPVHKN HHLCIEETFR GVDTLKAQIV QGEAMDNLFQ NLYLIKKYID LQKKKCGEER RGVKHFLDYL QEFLGVINTE WTMES (115)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight13.4 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Rabbit Interleukin-5 (IL-5) is a Th2-associated cytokine produced primarily by activated CD4⁺ T helper 2 (Th2) cells, mast cells, and type 2 innate lymphoid cells in rabbits (Oryctolagus cuniculus), where it plays a central role in eosinophil differentiation, activation, recruitment, and survival. IL-5 is a member of the common beta chain (βc) cytokine family, which also includes IL-3 and granulocyte–macrophage colony-stimulating factor (GM-CSF); these cytokines share the βc receptor subunit and regulate hematopoiesis and myeloid cell development. IL-5 signals through a receptor complex composed of IL-5Rα and the shared βc chain, activating JAK/STAT and MAPK pathways that promote eosinophil maturation in the bone marrow and enhance their effector functions in peripheral tissues. In rabbit health, IL-5 is particularly relevant in parasitic infections, allergic skin conditions, and respiratory inflammation, where elevated IL-5 contributes to eosinophilic infiltration, airway hyperreactivity, and tissue remodeling. Rabbits are widely used as experimental models for asthma and allergic airway disease, and IL-5 plays a key role in driving eosinophilic lung inflammation in these systems. While IL-5–mediated responses support protective immunity against helminths, excessive or sustained IL-5 signaling can promote chronic eosinophilic inflammation and fibrosis. As a biomarker of type 2 immune activation, rabbit IL-5 supports translational research into eosinophil biology, Th1/Th2 immune balance, and development of targeted anti–IL-5 therapies relevant to human allergic and inflammatory disorders.

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