Mouse EGF (Epidermal Growth Factor) is a growth factor peptide belonging to the EGF family of ligands, which also includes transforming growth factor-α (TGF-α), amphiregulin (AREG), heparin-binding EGF-like growth factor (HB-EGF), betacellulin (BTC), and epiregulin (EREG) that signal through the epidermal growth factor receptor (EGFR/ErbB1) to regulate cell proliferation, differentiation, and tissue repair. In mice (Mus musculus), EGF is produced by epithelial cells, fibroblasts, macrophages, and glandular tissues such as the submandibular (salivary) glands, which are a major source of circulating EGF in this species. Binding of EGF to EGFR activates intracellular signaling pathways including MAPK/ERK, PI3K–AKT, and JAK/STAT, promoting cell proliferation, survival, and tissue regeneration following injury or during development. Mouse EGF plays important roles in skin homeostasis, gastrointestinal epithelial maintenance, and organ development. Mice are one of the most widely used animal models for studying EGFR signaling and epithelial biology, and EGF has been extensively studied in mouse models of skin wound healing and epithelial regeneration, where EGFR-mediated signaling regulates keratinocyte proliferation, migration, and repair of damaged skin tissue. These studies have been critical for understanding growth factor signaling pathways and their roles in tissue repair and cancer biology.