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Canine EGF Recombinant Protein
- Catalog Number:
- RP1273D
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Canis lupus dingo (dingo), Canis lupus familiaris (dog), Halichoerus grypus (gray seal), Leptonychotes weddellii (Weddell seal), Vulpes lagopus (Arctic fox), Vulpes vulpes (red fox)
- Canine EGF (Epidermal Growth Factor) (catalog RP1273D) is a yeast-derived growth factor supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~6.2 kDa, 52 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to dog, dingo, Weddell seal, Arctic fox, red fox, and gray seal. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study EGF signaling and cellular processes (including regulation of cell proliferation, differentiation, and wound healing); typical experimental uses include cell-culture stimulation and dosing studies, growth and signaling assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | NGYRECPSSY DGYCLYNGVC MYIEAVDRYA CNCVFGYVGE RCQHRDLKWE LR (52) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 6.2 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Canine EGF (Epidermal Growth Factor) is a growth factor peptide belonging to the EGF family of ligands, which also includes transforming growth factor-α (TGF-α), amphiregulin (AREG), heparin-binding EGF-like growth factor (HB-EGF), betacellulin (BTC), and epiregulin (EREG) that signal through the epidermal growth factor receptor (EGFR/ErbB1) to regulate cell proliferation, differentiation, and tissue repair. In dogs (Canis lupus familiaris), EGF is produced by epithelial cells, fibroblasts, macrophages, and glandular tissues such as salivary and intestinal glands, where it contributes to maintenance and repair of epithelial tissues, including the skin, gastrointestinal tract, and cornea. Binding of EGF to EGFR activates intracellular signaling pathways such as MAPK/ERK, PI3K-AKT, and JAK/STAT, which promote cell proliferation, survival, and tissue regeneration following injury or inflammation. In canine health, EGF is important for wound healing and epithelial regeneration, particularly in cutaneous and corneal repair. Dogs are also used as comparative animal models for human dermatological and ocular diseases, and EGF signaling has been studied in canine models of corneal injury and wound healing, where EGFR-mediated pathways regulate epithelial regeneration and tissue repair, providing insights relevant to both veterinary medicine and human regenerative therapies.
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