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Swine IL-3 Recombinant Protein
- Catalog Number:
- RP1298S
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Sus scrofa (pig)
- Swine IL-3 (Interleukin-3) (catalog RP1298S) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~13.7 kDa, 121 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to pig. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study IL-3 signaling and hematopoiesis (including regulation of multipotent hematopoietic cell proliferation and differentiation); typical experimental uses include cell-culture stimulation and differentiation assays, cytokine signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | MPTTTLQPKN YLAMIQEITR SLENLTVTSN KSLTLNELET LVNNTLLRPN LEAFVTFAEN HLKNISGIKK NLEKFRPILP TSMSTEEPIS IEEGDLGDFR AKLMEYLVVL RDSLKPMITE P (121) Sequence based on published sequence. Hermanrud CE, et al., Expression and purification of non-N-glycosylated porcine interleukin 3 in yeast Pichia pastoris. Protein Expr Purif. 2012 Mar;82(1):70-4. doi: 10.1016/j.pep.2011.11.011. Epub 2011 Dec 1. |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 13.7 kda |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Swine interleukin-3 (IL-3) is a hematopoietic growth factor primarily produced by activated T cells and mast cells that supports the proliferation, differentiation, and survival of multipotent hematopoietic progenitors through signaling via the IL-3 receptor complex (IL-3Rα/CD123 and the common β chain), activating JAK/STAT, MAPK, and PI3K pathways. In swine health, IL-3 plays an important role in regulating myeloid cell development, including monocytes, macrophages, dendritic cells, basophils, and mast cells, thereby influencing innate immunity, inflammatory responses, and host defense against bacterial and viral infections relevant to production systems. Dysregulated IL-3 signaling may contribute to excessive inflammation, allergic-type responses, or altered immune cell dynamics during respiratory and enteric diseases that impact growth performance and herd productivity. Beyond its importance in livestock health, swine IL-3 is highly valuable in animal model research because pigs share close physiological, immunological, and hematopoietic similarities with humans, making them strong translational models for studying bone marrow biology, inflammatory disorders, sepsis, and immune cell development. As such, swine IL-3 supports both veterinary immunology applications and comparative biomedical research aimed at understanding hematopoiesis and immune regulation across species.
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