Bovine Basic Fibroblast Growth Factor (FGF2, also known as bFGF) is a heparin-binding growth factor belonging to the fibroblast growth factor (FGF) family, which includes more than 20 related proteins involved in cell proliferation, differentiation, angiogenesis, and tissue repair. In cattle (Bos taurus), FGF2 is produced by fibroblasts, endothelial cells, macrophages, and other stromal and epithelial cells, where it plays important roles in wound healing, vascular development, and tissue regeneration. Bovine FGF2 signals through fibroblast growth factor receptors (FGFR1–FGFR4) in the presence of heparan sulfate proteoglycans, activating intracellular pathways such as MAPK/ERK, PI3K–AKT, and PLCγ, which promote cell proliferation, migration, and angiogenesis. In bovine physiology, FGF2 contributes to development and repair of connective tissues, blood vessels, and muscle, and it is also involved in reproductive biology, including follicular development and ovarian function. Because of its strong ability to stimulate cell growth and angiogenesis, bovine FGF2 has been widely used in cell culture systems and stem cell research as a growth supplement to support proliferation and maintenance of mammalian cells, making it an important molecule in biotechnology, regenerative medicine, and veterinary research.