Slide 1 of 1 
Feline GM-CSF Recombinant Protein
- Catalog Number:
- RP1323F
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Felis catus (domestic cat)
- Feline GM-CSF (Granulocyte-Macrophage Colony-Stimulating Factor) (catalog RP1323F) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~14.5 kDa, 127 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to domestic cat. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study GM-CSF signaling and hematopoiesis (including regulation of granulocyte and macrophage differentiation and activation); typical experimental uses include cell-culture stimulation and differentiation assays, cytokine signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | APTSSPSSVT RPWQHVDAMK EALSLLNNSS EITAVMNETV EVVSEMFDPE EPKCLQTHLK LYEQGLRGSL ISLKEPLRMM ANHYKQHCPL TPETPCETQT ITFKNFKEKL KDFLFNNPFD CWGPDQK (127) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 14.5 kda |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Feline GM-CSF (Granulocyte-Macrophage Colony-Stimulating Factor, CSF2) is a hematopoietic cytokine belonging to the colony-stimulating factor family, which also includes G-CSF (CSF3) and M-CSF (CSF1) that regulate the development, proliferation, and activation of myeloid immune cells. In cats (Felis catus), GM-CSF is produced by activated T lymphocytes, macrophages, endothelial cells, and fibroblasts in response to infection, inflammatory cytokines, or immune stimulation. Feline GM-CSF binds to the GM-CSF receptor (CSF2R) expressed on hematopoietic progenitor cells and mature myeloid cells, activating intracellular signaling pathways such as JAK/STAT, MAPK/ERK, and PI3K-AKT, which promote the proliferation and differentiation of granulocytes, macrophages, and dendritic cells, as well as enhance their functional activity in host defense. In feline immunity, GM-CSF plays an important role in regulating innate immune responses and antigen presentation, particularly during viral and bacterial infections. Cats are also valuable animal models for human immunological diseases, and GM-CSF-regulated myeloid responses have been studied in feline immunodeficiency virus (FIV) infection, a widely used animal model for human HIV/AIDS, where GM-CSF influences macrophage activation and dendritic cell function, helping researchers understand immune dysregulation and antiviral immune responses relevant to both veterinary and human medicine.
Please choose a size to see price
RELATED MEDIA
