Multi-species (Human, Monkey) FGF basic Recombinant Protein

Catalog Number:
RP1324HY
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Aotus nancymaae (Ma's night monkey), Cercocebus atys (sooty mangabey), Chlorocebus sabaeus (green monkey), Colobus angolensis palliates (Peter’s Angolan Colobus), Gorilla gorilla gorilla (western lowland gorilla), Homo sapiens (human), Macaca fascicularis (crab-eating macaque), Macaca mulatta (Rhesus monkey), Macaca nemestrina (pig-tailed macaque), Mandrillus leucophaeus (drill), Pan paniscus (pygmy chimpanzee), Pan troglodytes (chimpanzee), Papio anubis (olive baboon), Pongo abelii (Sumatran orangutan), Rhinopithecus bieti (black snub-nosed monkey), Rhinopithecus roxellana (golden snub-nosed monkey), Saimiri boliviensis boliviensis (Bolivian squirrel monkey)
  • Human/Cynomolgus Monkey FGF basic (Fibroblast Growth Factor 2, FGF-2) (catalog RP1324HY) is a yeast-derived growth factor supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~16.3 kDa, 145 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to human, crab-eating macaque, Ma's night monkey, sooty mangabey, green monkey, Peter's Angolan colobus, western lowland gorilla, rhesus monkey, pig-tailed macaque, drill, pygmy chimpanzee, chimpanzee, olive baboon, Sumatran orangutan, black snub-nosed monkey, golden snub-nosed monkey, and Bolivian squirrel monkey. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study FGF signaling and cellular processes (including regulation of cell proliferation, differentiation, angiogenesis, and tissue repair); typical experimental uses include cell-culture stimulation and growth assays, signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceSLPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY SSWYVALKRT GQYKLGPKTG PGQKAILFLP MSAKS (145)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight16.3 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Affinity TagNone
aminoAcids145
Carrier ProteinNone
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Human and Cynomolgus Monkey Basic Fibroblast Growth Factor (FGF2, also known as bFGF) is a heparin-binding growth factor belonging to the fibroblast growth factor (FGF) family, which includes more than 20 related proteins involved in cell proliferation, differentiation, angiogenesis, and tissue repair. In humans (Homo sapiens) and cynomolgus monkeys (Macaca fascicularis), the FGF2 protein is identical at the amino acid level, reflecting strong evolutionary conservation and indicating highly similar biological functions in these primates. FGF2 is produced by fibroblasts, endothelial cells, macrophages, and other stromal and epithelial cells, where it regulates cell growth, tissue regeneration, and formation of new blood vessels. FGF2 signals through fibroblast growth factor receptors (FGFR1–FGFR4) in the presence of heparan sulfate proteoglycans, activating intracellular pathways such as MAPK/ERK, PI3K–AKT, and PLCγ, which promote cell proliferation, migration, and angiogenesis. In human biology, FGF2 is critical for wound healing, tissue repair, neurogenesis, and vascular development, and dysregulation of FGF2 signaling has been associated with cancer, cardiovascular disease, and abnormal tissue growth. Because cynomolgus monkeys share close genetic and physiological similarity with humans, they are widely used as preclinical animal models for regenerative medicine, angiogenesis, and cancer research, where the conserved FGF2 protein helps researchers evaluate therapeutic strategies targeting growth factor signaling pathways relevant to human disease.
  • Growth Factor

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