Rabbit FGF basic Recombinant Protein

Catalog Number:
RP1325U
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Oryctolagus cuniculus (rabbit)
  • Rabbit FGF basic (Fibroblast Growth Factor 2, FGF-2) (catalog RP1325U) is a yeast-derived growth factor supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~16.3 kDa, 145 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to rabbit. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study FGF signaling and cellular processes (including regulation of cell proliferation, differentiation, angiogenesis, and tissue repair); typical experimental uses include cell-culture stimulation and growth assays, signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY SSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS (145)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight16.3 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Rabbit Basic Fibroblast Growth Factor (FGF2, also known as bFGF) is a heparin-binding growth factor belonging to the fibroblast growth factor (FGF) family, which includes more than 20 related proteins involved in cell proliferation, differentiation, angiogenesis, and tissue repair. In rabbits (Oryctolagus cuniculus), FGF2 is produced by fibroblasts, endothelial cells, macrophages, and other stromal and epithelial cells, where it plays important roles in wound healing, vascular development, and tissue regeneration. FGF2 binds to fibroblast growth factor receptors (FGFR1–FGFR4) in the presence of heparan sulfate proteoglycans, activating intracellular signaling pathways such as MAPK/ERK, PI3K–AKT, and PLCγ, which promote cell proliferation, migration, survival, and angiogenesis. In rabbit physiology, FGF2 contributes to repair of connective tissues, skin regeneration, and formation of new blood vessels during healing processes. Rabbits are widely used as animal models for wound healing and angiogenesis research, and FGF2 has been studied in rabbit models of corneal injury and tissue repair, where FGF2-mediated signaling promotes corneal epithelial regeneration and neovascularization, providing insights into growth factor–driven tissue repair and regenerative therapies relevant to human medicine.

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