Cynomolgus Monkey CCL5 (RANTES) Recombinant Protein

Catalog Number:
RP1352Y
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Cercocebus atys (sooty mangabey), Macaca fascicularis (crab-eating macaque), Macaca mulatta (Rhesus monkey), Macaca nemestrina (pig-tailed macaque), Papio anubis (olive baboon)
  • Cynomolgus Monkey CCL5 (RANTES, Regulated upon Activation, Normal T Cell Expressed and Secreted) (catalog RP1352Y) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~7.8 kDa, 68 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to crab-eating macaque, sooty mangabey, rhesus monkey, pig-tailed macaque, and olive baboon. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study CCL5/RANTES signaling and immune-cell chemotaxis (including recruitment of T cells, eosinophils, and basophils during inflammatory responses); typical experimental uses include cell-culture stimulation and migration assays, chemotaxis and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceSPHASDTTPC CFAYIARPLP RAHIKEYFYT SGKCSNPAVV FVTRKNRQVC ANPEKKWVRE YINSLEMS (68)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight7.8 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Cynomolgus Monkey (Macaca fascicularis) CCL5 (C–C motif chemokine ligand 5, also known as RANTES – Regulated upon Activation, Normal T cell Expressed and Secreted) is a proinflammatory chemokine that regulates immune cell recruitment, antiviral responses, and inflammatory signaling in nonhuman primates. CCL5 belongs to the CC chemokine family, which also includes CCL3 (MIP-1α), CCL4 (MIP-1β), and CCL2 (MCP-1), chemokines that coordinate leukocyte trafficking during immune activation. In cynomolgus monkeys, CCL5 is produced primarily by activated T lymphocytes, macrophages, dendritic cells, platelets, epithelial cells, and endothelial cells in response to infection, inflammatory stimuli, or tissue injury. CCL5 exerts its biological effects mainly through binding to the chemokine receptors CCR1, CCR3, and CCR5, promoting chemotaxis and activation of T cells, monocytes/macrophages, eosinophils, and natural killer (NK) cells at sites of infection or inflammation. Because cynomolgus monkeys are widely used as preclinical animal models for human infectious diseases and immunology, CCL5 has been studied in models of viral infections such as HIV/SIV, dengue, and other emerging pathogens, where chemokine-mediated recruitment of immune cells plays an important role in antiviral immunity and inflammatory pathology. Due to their close genetic and immunological similarity to humans, cynomolgus monkey CCL5 is important for translational research on chemokine signaling, immune regulation, and development of immunomodulatory therapies targeting pathways such as CCR5-mediated leukocyte trafficking.

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