Bovine M-CSF Recombinant Protein

Catalog Number:: RP1353B
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Bison bison bison (American buffalo), Bos indicus (zebu), Bos mutus (wild yak), Bos taurus (cattle)

Product Description

Bovine M-CSF (Macrophage Colony-Stimulating Factor, CSF-1) (catalog RP1353B) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~27.2 kDa, 223 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to cattle, zebu, wild yak, and American buffalo. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study M-CSF/CSF-1 signaling and hematopoiesis (including regulation of macrophage differentiation, proliferation, and survival); typical experimental uses include cell-culture stimulation and differentiation assays, cytokine signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.

Specifications

Amino Acid Sequence	EEVSENCSHM IGNGHLLFLQ QLIDSQMETS CQISFEFVDQ EQLDDPVCYL KKAFLLVQDI MEDTMRFKDN TPNAKVIVQL QELSLRLKSC FTMDYDEQDK ACVRTFHETP LQLLEKIKNV FNETKNLLKK DWNIFSKNCN NSFAKCSSHD VVTKPDCNCL YPKATPSSDL ASVSPQQSPT PSTAPMAGLT WADSEATDGS SLLPREQALR TVDPGSAKQR PPR (223)
Endotoxin	Naturally endotoxin-free

Properties

Storage Conditions	-20°C
Molecular Weight	25.1 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Background Info

Bovine Macrophage Colony-Stimulating Factor (M-CSF) is a cytokine that regulates the development, proliferation, and survival of monocytes and macrophages in cattle (Bos taurus). M-CSF is produced by several cell types, including fibroblasts, endothelial cells, stromal cells, and epithelial cells, in response to tissue signals and inflammatory stimuli. Bovine M-CSF binds to the colony-stimulating factor-1 receptor (CSF1R), a receptor tyrosine kinase expressed on monocytes and macrophage lineage cells, activating intracellular signaling pathways such as PI3K-AKT, ERK/MAPK, and JAK-STAT that promote differentiation and maintenance of macrophage populations. Through these mechanisms, M-CSF supports macrophage-mediated immune surveillance, phagocytosis, tissue homeostasis, and inflammatory responses. Because macrophages play a central role in host defense and inflammatory regulation, M-CSF-mediated macrophage function is important for maintaining immune health and resistance to infection in cattle. In cell culture, recombinant bovine M-CSF is commonly used to promote differentiation of bovine monocytes into macrophages and to support macrophage proliferation and functional assays in vitro. Bovine M-CSF is also studied in animal model and veterinary research, including investigations of macrophage responses to important livestock pathogens such as Mycobacterium bovis, bovine viral diarrhea virus (BVDV), and Mycobacterium avium subsp. paratuberculosis, helping researchers better understand host-pathogen interactions and immune regulation in cattle.

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