Cynomolgus Monkey CCL19 (MIP-3 beta) Recombinant Protein

Catalog Number:
RP1357Y
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Cercocebus atys (sooty mangabey), Chlorocebus sabaeus (green monkey), Colobus angolensis palliates (Peter’s Angolan Colobus), Macaca fascicularis (crab-eating macaque), Macaca mulatta (Rhesus monkey), Macaca nemestrina (pig-tailed macaque), Mandrillus leucophaeus (drill), Papio anubis (olive baboon)
  • Cynomolgus Monkey CCL19 (MIP-3β) (catalog RP1357Y) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~8.8 kDa, 77 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with amino-acid homology to crab-eating macaque, sooty mangabey, green monkey, Peter's Angolan colobus, rhesus monkey, pig-tailed macaque, drill, and olive baboon. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study CCL19/MIP-3β signaling and immune-cell trafficking (including migration of dendritic cells and T cells to lymphoid tissues); typical experimental uses include cell-culture stimulation and migration assays, chemotaxis and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceGTNDAEDCCL SVTQKPIPGY IVRNFRYLLI KDGCRVPAVV FTTLRGRQLC APPDQSWVER IIQRLQRTST KMKRRSS (77)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight8.8 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Cynomolgus monkey CCL19 (C-C motif chemokine ligand 19), also known as macrophage inflammatory protein-3β (MIP-3β) or EBI1 ligand chemokine (ELC), is a member of the CC chemokine family that plays a central role in lymphocyte trafficking and organization of secondary lymphoid tissues in Cynomolgus monkeys (Macaca fascicularis). The CCL19 protein is highly conserved among non-human primates and is functionally comparable to that of Rhesus monkey (Macaca mulatta) and humans. CCL19 is produced primarily by stromal cells, dendritic cells, and macrophages within lymph nodes, spleen, and other lymphoid organs, where it signals through the CCR7 receptor to direct migration of naïve and central memory T cells, regulatory T cells, and mature dendritic cells. This CCR7–CCL19 axis is essential for antigen presentation, T cell priming, and maintenance of lymphoid architecture. In healthy Cynomolgus monkeys, CCL19 supports immune surveillance and adaptive immune organization; expression may increase during infection, vaccination, inflammatory stimulation, or immune-modulating biologic treatment. In preclinical and translational research, Cynomolgus monkey CCL19 is particularly relevant in studies of vaccine immunogenicity, autoimmune disease models, transplant immunology, and evaluation of immunotherapeutics, providing insight into CCR7-mediated immune cell trafficking and adaptive immune regulation in non-human primate models.

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