Cynomolgus Monkey IL-10 Recombinant Protein

Catalog Number:
RP1359Y
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Cercocebus atys (sooty mangabey), Chlorocebus sabaeus (green monkey), Macaca fascicularis (crab-eating macaque), Macaca mulatta (Rhesus monkey), Mandrillus leucophaeus (drill), Papio hamadryas (hamadryas baboon)
  • Cynomolgus Monkey IL-10 (Interleukin-10) (catalog RP1359Y) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~18.7 kDa, 160 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to crab-eating macaque, sooty mangabey, green monkey, rhesus monkey, drill, and hamadryas baboon. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study IL-10 signaling and immune regulation (including anti-inflammatory responses, inhibition of pro-inflammatory cytokine production, and regulation of immune-cell activity); typical experimental uses include cell-culture stimulation and inhibition studies, cytokine signaling assays, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceSPGQGTQSEN SCTRFPGNLP HMLRDLRDAF SRVKTFFQMK DQLDNILLKE SLLEDFKGYL GCQALSEMIQ FYLEEVMPQA ENHDPDIKEH VNSLGENLKT LRLRLRRCHR FLPCENKSKA VEQVKNAFSK LQEKGVYKAM SEFDIFINYI EAYMTMKIQN (160)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight18.7 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Cynomolgus Monkey (Macaca fascicularis) Interleukin-10 (IL-10) is a key anti-inflammatory cytokine produced by regulatory T cells (Tregs), Th2 cells, macrophages, dendritic cells, and certain B-cell populations, where it plays a central role in limiting excessive immune activation and maintaining immune homeostasis. Highly homologous to human IL-10, cynomolgus IL-10 signals through the IL-10 receptor complex (IL-10R1/IL-10R2), activating JAK/STAT3 pathways that suppress pro-inflammatory cytokine production (including IL-1β, TNF-α, and IFN-γ), reduce antigen presentation, and downregulate Th1-type cell-mediated responses. In nonhuman primate models, IL-10 is critically involved in modulating immune responses during infections such as dengue virus, influenza, SARS-CoV-2, simian immunodeficiency virus (SIV), tuberculosis, and bacterial sepsis, where elevated IL-10 levels can help prevent immunopathology but may also contribute to immune suppression and pathogen persistence. The IL-10/pro-inflammatory cytokine balance is frequently assessed in vaccine studies, immunotoxicology evaluations, and biologic safety testing. Because cynomolgus monkeys are widely used in preclinical research due to their close genetic and immunologic similarity to humans, characterization of IL-10 is essential for understanding immune regulation, cytokine network dynamics, and therapeutic modulation of inflammatory responses, providing critical translational insight into human infectious, autoimmune, and inflammatory diseases.

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