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Human PDGF-BB Recombinant Protein
- Catalog Number:
- RP1379H
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Homo sapiens (human)
- Human PDGF-BB (Platelet-Derived Growth Factor-BB) (catalog RP1379H) is a yeast-derived growth factor supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~12.3 kDa (monomer), 109 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to human. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study PDGF signaling and cellular processes (including regulation of cell proliferation, migration, and wound healing); typical experimental uses include cell-culture stimulation and proliferation assays, signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT (109) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 12.3 kDa (monomer) |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Human Platelet-Derived Growth Factor-BB (PDGF-BB) is a homodimeric growth factor composed of two PDGF-B chains that plays a critical role in cell proliferation, migration, angiogenesis, and tissue repair. PDGF-BB signals primarily through platelet-derived growth factor receptor-β (PDGFR-β) and PDGFR-α/β receptor complexes, activating intracellular signaling pathways including PI3K/AKT, MAPK, and STAT, which regulate fibroblast activation, smooth muscle cell proliferation, and extracellular matrix production. In humans, PDGF-BB is produced by platelets, macrophages, endothelial cells, fibroblasts, and other stromal cells, and is released during tissue injury, inflammation, and wound healing. It plays essential roles in vascular development, connective tissue repair, and regulation of mesenchymal cell growth, contributing to processes such as dermal wound healing and blood vessel formation. Dysregulated PDGF-BB signaling has been implicated in fibrotic diseases, atherosclerosis, and cancer, including glioblastoma, sarcomas, and other tumors, where PDGF-driven signaling can promote tumor cell proliferation and remodeling of the tumor microenvironment. Because of its central role in angiogenesis, tissue regeneration, and cellular growth signaling, human PDGF-BB is widely studied as both a biomarker and therapeutic target in regenerative medicine, fibrosis, vascular disease, and oncology.
5 ug
Price: $170.00
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