Atlantic Salmon IL-4-13 Recombinant Protein

Catalog Number:
RP1400AS
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Salmo salar (Atlantic salmon)
  • Atlantic Salmon IL-4/13 (Interleukin-4/13) (catalog RP1400AS) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~13.5 kDa, 117 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to Atlantic salmon. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study IL-4/13 signaling and immune responses (including regulation of Th2-type immune pathways, inflammation, and cytokine-mediated signaling); typical experimental uses include cell-culture stimulation and cytokine assays, signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceYRQHKNHHSN LEAIISMAEQ YTHNLTEDLL RTLLIDVTHL TGTTTKCKEF FCEAEKILAS VKNATFGNIV RHLRVYNKHQ YCIVVKSQDN QAEGNQVELR RLLLDLKECG QKINSKP (117)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight13.5 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Atlantic Salmon (Salmo salar) Interleukin-4/13 (IL-4/13)–like cytokines are type 2–associated immune mediators that share functional homology with mammalian IL-4 and IL-13 and play important roles in regulating humoral immunity, macrophage polarization, and mucosal inflammatory responses in teleost fish. Due to genome duplication events in salmonids, Atlantic salmon possess multiple IL-4/13–like paralogs that signal through receptor complexes homologous to IL-4Rα/IL-13R–type chains, activating STAT6-dependent pathways that promote alternative (M2-like) macrophage activation, modulation of pro-inflammatory cytokine production, and support of epithelial barrier function. In salmon health, IL-4/13–like cytokines are particularly relevant in responses to parasitic infections such as Lepeophtheirus salmonis (sea lice), as well as bacterial pathogens like Aeromonas salmonicida and Piscirickettsia salmonis, where balanced type 2 responses contribute to tissue repair and regulation of excessive inflammation. Dysregulated expression may influence susceptibility to chronic inflammation or impaired pathogen clearance. As biomarkers of type 2–like immune polarization, Atlantic salmon IL-4/13–like cytokines are important in vaccine development, aquaculture disease management, and studies of host–pathogen interactions. Characterization of these cytokines supports comparative immunology research into the evolution of Th2-type immunity and informs strategies to enhance disease resistance and fish welfare in commercial aquaculture systems.

Please choose a size to see price


Reagents for Animal Model and Animal Health Research

Kingfisher Biotech, Inc.
1000 Westgate Drive
Suite 123
Saint Paul, MN 55114

Phone: 651-646-0089
Fax: 651-646-0095

Animal Antibodies

Newsletter

"