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Human SCF (Stem Cell Factor) Recombinant Protein
- Catalog Number:
- RP1405H
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Chlorocebus sabaeus (green monkey), Gorilla gorilla gorilla (western lowland gorilla), Homo sapiens (human), Macaca nemestrina (pig-tailed macaque), Mandrillus leucophaeus (drill), Nomascus leucogenys (northern white-cheeked gibbon), Pan paniscus (pygmy chimpanzee), Pan troglodytes (chimpanzee), Papio anubis (olive baboon), Pongo abelii (Sumatran orangutan)
- Human SCF (Stem Cell Factor, KIT Ligand, KITLG) (catalog RP1405H) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~18.5 kDa, 165 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to human, green monkey, western lowland gorilla, pig-tailed macaque, drill, northern white-cheeked gibbon, pygmy chimpanzee, chimpanzee, olive baboon, and Sumatran orangutan. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study SCF/KIT signaling and hematopoiesis (including regulation of stem cell maintenance, proliferation, and differentiation); typical experimental uses include cell-culture stimulation and differentiation assays, cytokine signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | EGICRNRVTN NVKDVTKLVA NLPKDYMITL KYVPGMDVLP SHCWISEMVV QLSDSLTDLL DKFSNISEGL SNYSIIDKLV NIVDDLVECV KENSSKDLKK SFKSPEPRLF TPEEFFRIFN RSIDAFKDFV VASETSDCVV SSTLSPEKDS RVSVTKPFML PPVAA (165) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 18.8 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Human Stem Cell Factor (SCF) (also known as KIT ligand, KITLG, mast cell growth factor, or steel factor) is a cytokine and growth factor that plays a central role in hematopoiesis, stem cell maintenance, and immune cell development. SCF belongs to a group of hematopoietic growth factors that regulate the survival, proliferation, and differentiation of stem and progenitor cells. It functions by binding to the c-Kit receptor (CD117), a receptor tyrosine kinase expressed on hematopoietic stem cells, mast cells, melanocytes, and germ cells, activating intracellular signaling pathways including PI3K/AKT, MAPK, and JAK/STAT that promote cell survival, proliferation, and differentiation. In humans, SCF is produced by bone marrow stromal cells, fibroblasts, endothelial cells, epithelial cells, and other supporting cells, and exists in both membrane-bound and soluble forms, allowing both local and systemic regulation of c-Kit-expressing cells. SCF is essential for hematopoietic stem cell maintenance within the bone marrow niche, as well as for mast cell development, melanocyte function, and germ cell survival. Dysregulation of SCF-c-Kit signaling has been implicated in several diseases, including mastocytosis, gastrointestinal stromal tumors (GIST), melanoma, and certain leukemias, where abnormal activation of the c-Kit pathway can drive uncontrolled cell proliferation. Because of its critical role in stem cell biology, hematopoiesis, and oncogenic signaling, human SCF is widely studied in hematology, cancer biology, immunology, and regenerative medicine, contributing to development of therapies targeting the SCF-c-Kit signaling axis.
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