Bovine CTLA-4 Recombinant Protein

Catalog Number:: RP1411B
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Bison bison bison (American buffalo), Bos grunniens (domestic yak), Bos indicus (zebu), Bos mutus (wild yak), Bos taurus (cattle), Bubalus bubalis (water buffalo), Bubalus carabanensis (carabao)

Bovine CTLA-4 (Cytotoxic T-Lymphocyte-Associated Protein 4, CD152) (catalog RP1411B) is a yeast-derived immune checkpoint protein supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~13.8 kDa, 128 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to cattle, American buffalo, domestic yak, zebu, wild yak, water buffalo, and carabao. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study CTLA-4 signaling and immune regulation (including inhibition of T-cell activation and modulation of immune checkpoint pathways); typical experimental uses include cell-culture stimulation and binding assays, immune checkpoint and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.

Amino Acid Sequence	FSKGMNVTQP PVVLASSRGV ASFSCEYESS GKADEVRVTV LREAGSQVTE VCAGTYMVED ELTFLDDSTC IGTSRGNKVN LTIQGLRAMD TGLYVCKVEL MYPPPYYVGI GNGTQIYVID PEPCPDSD (128)
Endotoxin	Naturally endotoxin-free

Storage Conditions	-20°C
Molecular Weight	13.8 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Bovine CTLA-4 (Cytotoxic T-Lymphocyte-Associated Protein 4, also known as CD152) is an immune checkpoint receptor belonging to the immunoglobulin superfamily that plays a critical role in the negative regulation of T-cell activation in cattle (Bos taurus). CTLA-4 is primarily expressed on activated CD4⁺ and CD8⁺ T lymphocytes and regulatory T cells (Tregs) following antigen stimulation. It functions by binding to the costimulatory ligands CD80 (B7-1) and CD86 (B7-2) on antigen-presenting cells such as dendritic cells, macrophages, and B cells, competing with the activating receptor CD28 for these ligands. Through this interaction, CTLA-4 transmits inhibitory signals that reduce T-cell proliferation, cytokine production, and immune activation, helping to maintain immune homeostasis and prevent excessive inflammation. In bovine immunology, CTLA-4 plays an important role in regulating immune responses to viral, bacterial, and parasitic infections, as well as in controlling immune-mediated inflammation during diseases affecting cattle. Because immune checkpoint pathways are essential for balancing immune activation and tolerance, bovine CTLA-4 is studied in veterinary immunology, infectious disease research, and vaccine response studies, contributing to improved understanding of T-cell regulation and immune modulation in livestock health.

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