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Human EGF Recombinant Protein
- Catalog Number:
- RP1424H
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Gorilla gorilla gorilla (western lowland gorilla), Homo sapiens (human), Pan paniscus (pygmy chimpanzee), Pan troglodytes (chimpanzee)
- Human EGF (Epidermal Growth Factor) (catalog RP1424H) is a yeast-derived growth factor supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~6.2 kDa, 53 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to human, pygmy chimpanzee, chimpanzee, and western lowland gorilla. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study EGF signaling and cellular processes (including regulation of cell proliferation, differentiation, and survival); typical experimental uses include cell-culture stimulation and growth assays, signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR (53) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 6.2 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Human EGF (Epidermal Growth Factor) is a growth factor peptide belonging to the EGF family of ligands, which also includes transforming growth factor-α (TGF-α), amphiregulin (AREG), heparin-binding EGF-like growth factor (HB-EGF), betacellulin (BTC), and epiregulin (EREG) that signal through the epidermal growth factor receptor (EGFR/ErbB1) to regulate cell proliferation, differentiation, and tissue repair. In humans (Homo sapiens), EGF is produced by epithelial cells, fibroblasts, macrophages, and glandular tissues, particularly the salivary glands and gastrointestinal epithelium, where it plays important roles in maintenance and regeneration of epithelial tissues, including the skin, cornea, and intestinal lining. Binding of EGF to EGFR activates intracellular signaling pathways such as MAPK/ERK, PI3K-AKT, and JAK/STAT, promoting cell proliferation, survival, migration, and tissue remodeling during development and after injury. In human health, EGF is critical for wound healing, epithelial barrier maintenance, and tissue regeneration, and dysregulation of EGF-EGFR signaling is strongly associated with cancer development and progression, particularly in lung, colorectal, and head and neck cancers. Consequently, the EGF-EGFR signaling axis is a major focus of biomedical research and targeted cancer therapy, with EGFR inhibitors widely used as therapeutic agents in oncology.
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