Atlantic Salmon IL-8 Recombinant Protein

Catalog Number:
RP1425AS
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Salmo salar (Atlantic salmon)
  • Atlantic Salmon IL-8 (CXCL8, Interleukin-8) (catalog RP1425AS) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~8.5 kDa, 75 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to Atlantic salmon. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study IL-8/CXCL8 signaling and immune-cell chemotaxis (including recruitment and activation of neutrophil-like cells during inflammatory responses); typical experimental uses include cell-culture stimulation and migration assays, chemotaxis and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceMSLRGVGADL RCRCIETESR RIGRLIKKVE MFPPSSHCRD TEIIATLSNS GQEICLDVSA PWVKRVIKKM LANNK (75)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight8.5 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Atlantic Salmon (Salmo salar) Interleukin-8 (IL-8), a member of the CXC chemokine family, is a key pro-inflammatory mediator that regulates leukocyte recruitment and activation in teleost fish. Functionally analogous to mammalian IL-8, salmon IL-8 is rapidly induced in response to bacterial pathogens such as Aeromonas salmonicida and Vibrio species, viral infections including infectious salmon anemia virus (ISAV) and piscine orthoreovirus (PRV), and parasitic challenges such as sea lice (Lepeophtheirus salmonis). Expressed in immune-relevant tissues including gill, skin, spleen, kidney, and intestine, IL-8 promotes chemotaxis of neutrophil-like granulocytes and macrophages to sites of infection or tissue damage, supporting early innate immune defense. Elevated IL-8 expression is associated with localized inflammation and disease severity, making it a valuable biomarker for immune activation and pathogen exposure in aquaculture systems. In research and industry settings, characterization of Atlantic salmon IL-8 supports studies of mucosal immunity, vaccine efficacy, stress physiology, and host-pathogen interactions in aquatic environments, contributing to improved disease management, selective breeding for resistance, and sustainable aquaculture practices.

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