Swine Angiogenin-1 (Ang-1) Recombinant Protein

Catalog Number:
RP1445S
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Sus scrofa (pig)
  • Swine Ang-1 (Angiopoietin-1) (catalog RP1445S) is a yeast-derived growth factor supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~14.1 kDa, 123 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to pig. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study Ang-1 signaling and vascular biology (including regulation of angiogenesis, vascular stability, and endothelial cell survival); typical experimental uses include cell-culture stimulation and angiogenesis assays, signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceKDEDRYTHFL TQHYDAKPKG RDGRYCESIM KQRGLTRPCK EVNTFIHGTR NDIKAICNDK NGEPYNNFRR SKSPFQITTC KHKGGSNRPP CGYRATAGFR TIAVACENGL PVHFDESFII TSQ (123)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight14.1 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Swine angiogenin (ANG) is a secreted ribonuclease belonging to the RNase A superfamily that plays a critical role in angiogenesis, tissue remodeling, and cellular stress responses. In pigs (Sus scrofa), angiogenin is produced by endothelial cells, epithelial cells, macrophages, and various stromal cell types, where it promotes endothelial cell proliferation, migration, and capillary formation through activation of pro-angiogenic signaling pathways and stimulation of ribosomal RNA transcription. Beyond its vascular effects, swine angiogenin is involved in cellular survival under hypoxic or inflammatory stress and may contribute to regulation of innate immune responses and tissue repair processes. In healthy pigs, circulating angiogenin levels are typically low to moderate, but expression may increase during wound healing, ischemia, inflammation, tumor development, and experimental models of cardiovascular or metabolic disease. In veterinary and translational research, swine angiogenin is of particular interest in models of myocardial infarction, peripheral vascular disease, wound healing, surgical repair, and oncology, given the physiological and cardiovascular similarities between pigs and humans. Characterizing angiogenin expression in swine provides valuable insight into mechanisms of neovascularization, regenerative biology, and angiogenesis-driven pathology in both agricultural and biomedical research contexts.

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