Mouse Angiogenin-1 (Ang-1) Recombinant Protein

Catalog Number:
RP1469M
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Mus musculus (house mouse)
  • Mouse Ang-1 (Angiopoietin-1) (catalog RP1469M) is a yeast-derived growth factor supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~13.7 kDa, 121 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to house mouse. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study Ang-1 signaling and vascular biology (including regulation of angiogenesis, vascular stability, and endothelial cell survival); typical experimental uses include cell-culture stimulation and angiogenesis assays, signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceQDDSRYTKFL TQHHDAKPKG RDDRYCERMM KRRSLTSPCK DVNTFIHGNK SNIKAICGAN GSPYRENLRM SKSPFQVTTC KHTGGSPRPP CQYRASAGFR HVVIACENGL PVHFDESFFS L (121)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight13.7 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Mouse angiogenin (ANG) refers to members of the murine angiogenin family within the RNase A superfamily and plays important roles in angiogenesis, tissue remodeling, and cellular stress responses. Unlike humans, mice possess multiple angiogenin paralogs (e.g., Ang1–Ang4), which are expressed in a tissue-specific manner and contribute to endothelial cell proliferation, migration, and capillary formation through activation of pro-angiogenic signaling pathways and stimulation of ribosomal RNA transcription. In addition to its vascular functions, mouse angiogenin participates in regulation of cellular survival during hypoxic or inflammatory stress and has been implicated in host defense and gut mucosal biology, particularly Ang4, which is expressed in Paneth cells. Baseline expression in healthy mice varies by tissue but is generally low to moderate in circulation, with increased levels observed during wound healing, ischemia, tumor growth, inflammation, and experimental vascular injury. In biomedical research, mouse angiogenin is widely studied in models of cancer, atherosclerosis, myocardial infarction, peripheral vascular disease, inflammatory bowel disease, and neurodegeneration. Characterizing angiogenin expression in mice provides valuable insight into mechanisms of neovascularization, tissue regeneration, immune modulation, and angiogenesis-driven disease processes in experimental models.

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