Canine EGF (Epidermal Growth Factor) is a growth factor peptide belonging to the EGF family of ligands, which also includes transforming growth factor-α (TGF-α), amphiregulin (AREG), heparin-binding EGF-like growth factor (HB-EGF), betacellulin (BTC), and epiregulin (EREG) that signal through the epidermal growth factor receptor (EGFR/ErbB1) to regulate cell proliferation, differentiation, and tissue repair. In dogs (Canis lupus familiaris), EGF is produced by epithelial cells, fibroblasts, macrophages, and glandular tissues such as salivary and intestinal glands, where it contributes to maintenance and repair of epithelial tissues, including the skin, gastrointestinal tract, and cornea. Binding of EGF to EGFR activates intracellular signaling pathways such as MAPK/ERK, PI3K–AKT, and JAK/STAT, which promote cell proliferation, survival, and tissue regeneration following injury or inflammation. In canine health, EGF is important for wound healing and epithelial regeneration, particularly in cutaneous and corneal repair. Dogs are also used as comparative animal models for human dermatological and ocular diseases, and EGF signaling has been studied in canine models of corneal injury and wound healing, where EGFR-mediated pathways regulate epithelial regeneration and tissue repair, providing insights relevant to both veterinary medicine and human regenerative therapies.