Human CXCL2 Recombinant Protein

Catalog Number:
RP1510H
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Gorilla gorilla gorilla (western lowland gorilla), Homo sapiens (human)
  • Human CXCL2 (MIP-2α, Macrophage Inflammatory Protein-2 alpha) (catalog RP1510H) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~7.9 kDa, 73 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to human and western lowland gorilla. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study CXCL2/MIP-2α signaling and immune-cell chemotaxis (including recruitment and activation of neutrophils during inflammatory responses); typical experimental uses include cell-culture stimulation and migration assays, chemotaxis and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceAPLATELRCQ CLQTLQGIHL KNIQSVKVKS PGPHCAQTEV IATLKNGQKA CLNPASPMVK KIIEKMLKNG KSN (73)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight7.9 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Human CXCL2 (C-X-C motif chemokine ligand 2), also known as MIP-2α (macrophage inflammatory protein-2 alpha) or GRO-β (growth-regulated oncogene beta), is a proinflammatory chemokine belonging to the CXC chemokine family, which also includes CXCL1 (GRO-α) and CXCL3 (GRO-γ) that regulate neutrophil recruitment during inflammatory responses. In humans (Homo sapiens), CXCL2 is produced by macrophages, monocytes, epithelial cells, endothelial cells, and fibroblasts in response to proinflammatory cytokines such as IL-1β and TNF-α, microbial products, or tissue injury. CXCL2 primarily signals through the chemokine receptor CXCR2, which is expressed on neutrophils and other innate immune cells, promoting chemotaxis, activation, and migration of neutrophils to sites of infection or inflammation. Through these actions, CXCL2 plays a key role in innate immune defense against bacterial pathogens and in the regulation of acute inflammatory responses, particularly in tissues such as the lung, skin, and gastrointestinal tract. Dysregulated CXCL2 expression has been implicated in chronic inflammatory diseases, tumor-associated inflammation, and cancer progression, where CXCL2-mediated neutrophil recruitment can influence tissue inflammation and the tumor microenvironment. As a result, human CXCL2 is widely studied in immunology, infectious disease research, and cancer biology as both a mediator of inflammatory signaling and a potential therapeutic target.

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