Dolphin CCL2 (MCP-1) Recombinant Protein

Catalog Number:
RP1513P
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Lagenorhynchus obliquidens (Pacific white-sided dolphin), Orcinus orca (killer whale), Tursiops truncatus (bottlenose dolphin)
  • Dolphin CCL2 (MCP-1, Monocyte Chemoattractant Protein-1) (catalog RP1513P) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~8.8 kDa, 76 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to bottlenose dolphin, Pacific white-sided dolphin, and killer whale. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study CCL2/MCP-1 signaling and immune-cell chemotaxis (including recruitment of monocytes and other leukocytes during inflammatory responses); typical experimental uses include cell-culture stimulation and migration assays, chemotaxis and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceQPDAINSPVT CCYTFANKKI SNQRLMSYRR VTSSKCPKEA VIFKTILGKD ICADPKQKWV QDSMRHLDKK RQTPKP (76)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight8.8 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Dolphin CCL2 (C-C motif chemokine ligand 2, also known as monocyte chemoattractant protein-1, MCP-1) is a pro-inflammatory chemokine that regulates recruitment and activation of monocytes, macrophages, and memory T cells during immune responses in dolphins, particularly the bottlenose dolphin (Tursiops truncatus). CCL2 primarily signals through the CCR2 receptor, activating intracellular pathways that promote leukocyte migration, cytokine production, and coordination of innate and adaptive immune responses at sites of infection, inflammation, or tissue injury. In dolphin health, CCL2 is believed to play an important role in inflammatory responses associated with respiratory infections, skin lesions, parasitic disease, and wound healing, where monocyte and macrophage recruitment contributes to pathogen clearance and tissue repair. Dysregulated or prolonged CCL2 expression may contribute to chronic inflammatory conditions or fibrosis, especially in animals exposed to environmental stressors such as pollutants, harmful algal toxins, or infectious outbreaks. Because dolphins are considered sentinel species for marine ecosystem health, CCL2 expression is an important biomarker for studying immune activation, environmental immunotoxicology, and host–pathogen interactions in marine mammals. Characterization of dolphin CCL2 therefore supports research into chemokine-mediated immune regulation, marine wildlife health, and comparative inflammatory biology across mammalian species.

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