Multi-species (Ovine, Caprine) CCL2 (MCP-1) Recombinant Protein

Catalog Number:: RP1514V
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Capra hircus (goat), Ovis aries (sheep)

Product Description

Ovine/Caprine CCL2 (MCP-1, Monocyte Chemoattractant Protein-1) (catalog RP1514V) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~8.7 kDa, 75 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to goat and sheep. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study CCL2/MCP-1 signaling and immune-cell chemotaxis (including recruitment of monocytes and other leukocytes during inflammatory responses); typical experimental uses include cell-culture stimulation and migration assays, chemotaxis and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.

Specifications

Amino Acid Sequence	QPDAINSQIA CCYKFNKKIP IQRLTNYRRV TTSKCPKEAV IFKTILGKEF CADPNLKWVQ DAINHLNKKN QTPKP (75)
Endotoxin	Naturally endotoxin-free

Properties

Storage Conditions	-20°C
Molecular Weight	8.7 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Background Info

Ovine (Ovis aries) and Caprine (Capra hircus) CCL2 (C-C motif chemokine ligand 2, also known as monocyte chemoattractant protein-1, MCP-1) are highly conserved chemokines that play a key role in recruitment and activation of monocytes, macrophages, and memory T cells during immune responses in small ruminants. Because the ovine and caprine proteins are identical in sequence, they exhibit the same functional properties and receptor interactions. CCL2 primarily signals through the CCR2 receptor, activating intracellular pathways that promote leukocyte migration, cytokine production, and coordination of innate and adaptive immune responses at sites of infection, inflammation, or tissue injury. In sheep and goats, CCL2 is particularly important in inflammatory and infectious diseases such as mastitis, pneumonia, gastrointestinal parasitic infections, and caseous lymphadenitis, where recruitment of monocytes and macrophages is essential for pathogen control and tissue repair. However, excessive or prolonged CCL2 expression may contribute to chronic inflammation and tissue damage. As a biomarker of monocyte/macrophage recruitment and inflammatory activation, ovine and caprine CCL2 are widely studied in investigations of host-pathogen interactions, vaccine responses, and immune regulation in small ruminant production systems. Characterization of CCL2 in these species supports veterinary research aimed at improving disease resistance, understanding inflammatory disease mechanisms, and enhancing the health and productivity of sheep and goats.

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