Feline Erythropoietin (EPO) Recombinant Protein

Catalog Number:: RP1524F
Application:: Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:: Felis catus (domestic cat)

Product Description

Feline EPO (Erythropoietin) (catalog RP1524F) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~18.3 kDa, 166 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to domestic cat. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study EPO signaling and hematopoiesis (including regulation of erythroid progenitor proliferation, differentiation, and survival); typical experimental uses include cell-culture stimulation and differentiation assays, cytokine signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.

Properties/Specifications

Amino Acid Sequence	APPRLICDSR VLERYILGAR EAENVTMGCA EGCSFSENIT VPDTKVNFYT WKRMDVGQQA VEVWQGLALL SEAILRGQAL LANSSQPSET LQLHVDKAVS SLRSLTSLLR ALGAQKEATS LPEATSAAPL RTFTVDTLCK LFRIYSNFLR GKLTLYTGEA CRRGDR (166)
Endotoxin	Naturally endotoxin-free
Form	Lyophilized
Storage Conditions	-20°C
Molecular Weight	18.3 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Affinity Tag	None
aminoAcids	166
Carrier Protein	None
Expression System	Yeast
Form	Lyophilized
Country Of Origin	USA

Feline Erythropoietin (EPO) is a glycoprotein hormone belonging to the type I cytokine family that regulates erythropoiesis, the production of red blood cells. In cats (Felis catus), erythropoietin is produced primarily by peritubular interstitial cells in the kidney, with smaller amounts produced in the liver, in response to hypoxia or reduced oxygen-carrying capacity of the blood. Feline EPO binds to the erythropoietin receptor (EPOR) on erythroid progenitor cells in the bone marrow, activating signaling pathways such as JAK2–STAT5, PI3K–AKT, and MAPK, which promote survival, proliferation, and differentiation of erythroid precursor cells into mature red blood cells. In feline health, erythropoietin is essential for maintaining normal hematocrit and oxygen delivery to tissues, and insufficient EPO production is a major contributor to anemia associated with chronic kidney disease (CKD), a common condition in aging cats. Cats also serve as clinical models for studying anemia of chronic kidney disease, and research on feline erythropoietin has contributed to understanding the pathophysiology of renal anemia and the therapeutic use of recombinant erythropoietin or EPO-stimulating agents to manage anemia in both veterinary and human medicine.