Caprine CCL3 (MIP-1 alpha) Recombinant Protein

Catalog Number:
RP1525G
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Capra hircus (goat)
  • Caprine CCL3 (MIP-1α, Macrophage Inflammatory Protein-1 alpha) (catalog RP1525G) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~7.8 kDa, 70 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to goat. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study CCL3/MIP-1α signaling and immune-cell chemotaxis (including recruitment and activation of monocytes, T cells, and other leukocytes during inflammatory responses); typical experimental uses include cell-culture stimulation and migration assays, chemotaxis and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceAPFGADTPTA CCFSYAARQL SRKIIADYFE TSSQCSKPGV IFQTKRGRQV CANPSEDWVQ EYVTDLELNS (70)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight7.8 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Caprine CCL3 (C-C motif chemokine ligand 3, also known as MIP-1α, macrophage inflammatory protein-1 alpha) is a pro-inflammatory chemokine that plays an important role in recruitment and activation of immune cells during inflammatory and infectious responses in goats (Capra hircus). CCL3 primarily signals through the CCR1 and CCR5 receptors, promoting chemotaxis of monocytes, macrophages, dendritic cells, natural killer (NK) cells, and activated T lymphocytes to sites of infection or tissue injury. In goats, CCL3 is produced by activated macrophages, dendritic cells, epithelial cells, and lymphocytes in response to inflammatory stimuli such as microbial components and cytokines including TNF-α and IL-1β. CCL3-mediated immune cell recruitment is important for host defense against pathogens involved in diseases such as caprine arthritis encephalitis virus (CAEV) infection, mastitis, and respiratory or bacterial infections, where infiltration of monocytes and lymphocytes contributes to immune activation and pathogen clearance. While CCL3-driven leukocyte trafficking supports protective immune responses, excessive or prolonged expression may contribute to chronic inflammation and tissue damage. As a regulator of immune cell migration and inflammatory signaling, caprine CCL3 is important in studies of host–pathogen interactions, inflammatory disease mechanisms, and immune responses relevant to goat health and small ruminant veterinary research.

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Saint Paul, MN 55114

Phone: 651-646-0089
Fax: 651-646-0095

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