Turkey CCL3 (MIP-1 alpha) Recombinant Protein

Catalog Number:
RP1526T
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Meleagris gallopavo (turkey)
  • Turkey CCL3 (MIP-1α, Macrophage Inflammatory Protein-1 alpha) (catalog RP1526T) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~13.5 kDa, 124 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to turkey. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study CCL3/MIP-1α signaling and immune responses (including regulation of leukocyte recruitment and inflammatory processes); typical experimental uses include cell-culture stimulation and migration assays, chemotaxis and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceIKVAAQQRPS IVQSRALQPP SSSPAHCANV LQTMKGPAAI LTALLLLALC SSAVAHLDEL PTTCCFSYVQ RPIPRNIIKS AYITSSKCRL PAVILVTKKG KEICANPEES WVQNHLELFQ NEEN (124)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight13.5 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Turkey CCL3 (C-C motif chemokine ligand 3, also known as MIP-1α, macrophage inflammatory protein-1 alpha) is a pro-inflammatory chemokine involved in recruitment and activation of immune cells during inflammatory and infectious responses in turkeys (Meleagris gallopavo). CCL3 primarily signals through chemokine receptors homologous to mammalian CCR1 and CCR5, promoting chemotaxis of monocytes, macrophages, dendritic cells, natural killer–like cells, and activated T lymphocytes to sites of infection or tissue injury. In turkeys, CCL3 is produced by activated macrophages, epithelial cells, and lymphocytes in response to inflammatory stimuli such as pathogen-associated molecules and cytokines including IL-1β and TNF-like cytokines. CCL3-mediated immune cell recruitment plays an important role in host defense against pathogens associated with poultry respiratory and systemic diseases, including avian influenza virus, Newcastle disease virus, and bacterial infections such as Mycoplasma gallisepticum. While CCL3-driven leukocyte trafficking supports pathogen clearance and activation of immune responses, excessive or prolonged expression may contribute to inflammatory tissue damage. As a regulator of immune cell migration and inflammatory signaling, turkey CCL3 is important for studies of avian host–pathogen interactions, immune regulation, and vaccine responses, supporting research aimed at improving disease resistance and health in commercial poultry production.

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