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Human CX3CL1 (Fractalkine) Recombinant Protein
- Catalog Number:
- RP1527H
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Gorilla gorilla gorilla (western lowland gorilla), Homo sapiens (human), Pan paniscus (pygmy chimpanzee), Pan troglodytes (chimpanzee) (green monkey)
- Human CX3CL1 (Fractalkine) (catalog RP1527H) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~8.6 kDa, 76 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to human, pygmy chimpanzee, chimpanzee, green monkey, and western lowland gorilla. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study CX3CL1/fractalkine signaling and immune-cell adhesion and migration (including regulation of leukocyte trafficking, adhesion to endothelial cells, and inflammatory responses); typical experimental uses include cell-culture stimulation and adhesion/migration assays, chemotaxis and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | QQHGVNKCNL FCNKMTQKIP ESRLVGYQRN RESCNDGAVI LKTVKGKSFC ADPKEEWVQK AMKHLDHKAS VSQKSG (76) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 8.6 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Human CX3CL1 (C-X3-C motif chemokine ligand 1), also known as fractalkine, is a chemokine belonging to the CX3C chemokine family, characterized by three amino acids separating the first two conserved cysteine residues. CX3CL1 is unique among chemokines because it exists in both a membrane-bound form and a soluble form, allowing it to function in leukocyte adhesion and chemotaxis. In humans (Homo sapiens), CX3CL1 is produced primarily by endothelial cells, epithelial cells, neurons, and macrophages in response to inflammatory cytokines, infection, or tissue injury. CX3CL1 binds to its receptor CX3CR1, which is expressed on monocytes, macrophages, natural killer (NK) cells, cytotoxic T lymphocytes, and microglia, promoting adhesion, migration, and activation of these immune cells at sites of inflammation. In human physiology, the CX3CL1-CX3CR1 signaling pathway plays important roles in immune surveillance, neuroimmune communication, and vascular inflammation. Dysregulation of this pathway has been implicated in chronic inflammatory diseases, neurodegenerative disorders such as Alzheimer's disease, and cardiovascular diseases including atherosclerosis, where fractalkine-mediated monocyte recruitment contributes to vascular plaque formation. Because of its role in leukocyte trafficking and inflammatory signaling, human CX3CL1 is widely studied in immunology, neurobiology, and cardiovascular disease research, and is considered a potential therapeutic target for inflammatory and neurodegenerative diseases.
5 ug
Price: $170.00
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