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Human CCL20 Recombinant Protein
- Catalog Number:
- RP1528H
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Gorilla gorilla gorilla (western lowland gorilla), Homo sapiens (human), Pan paniscus (pygmy chimpanzee), Pan troglodytes (chimpanzee)
- Human CCL20 (MIP-3α, Macrophage Inflammatory Protein-3 alpha) (catalog RP1528H) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~8.0 kDa, 70 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to human, western lowland gorilla, pygmy chimpanzee, and chimpanzee. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study CCL20/MIP-3α signaling and immune-cell trafficking (including recruitment of lymphocytes and dendritic cells via CCR6 during inflammatory and mucosal immune responses); typical experimental uses include cell-culture stimulation and migration assays, chemotaxis and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | ASNFDCCLGY TDRILHPKFI VGFTRQLANE GCDINAIIFH TKKKLSVCAN PKQTWVKYIV RLLSKKVKNM (70) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 8.0 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Human CCL20 (C-C motif chemokine ligand 20, also known as MIP-3α, macrophage inflammatory protein-3 alpha, or LARC - liver and activation-regulated chemokine) is a pro-inflammatory chemokine that plays a key role in mucosal immune defense and leukocyte trafficking. CCL20 signals specifically through the CCR6 receptor, promoting chemotaxis of immune cells including immature dendritic cells, memory T cells, Th17 cells, regulatory T cells, and certain B cell populations to sites of epithelial infection or inflammation. In humans, CCL20 is produced by epithelial cells, fibroblasts, endothelial cells, and immune cells in response to inflammatory stimuli such as microbial products, cytokines (e.g., IL-1β, TNF-α, and IL-17), and tissue injury. It is highly expressed at mucosal surfaces, including the intestinal tract, respiratory epithelium, skin, and reproductive tract, where it contributes to recruitment of antigen-presenting cells and initiation of adaptive immune responses. Dysregulated CCL20 expression is implicated in several chronic inflammatory and autoimmune diseases, including inflammatory bowel disease, psoriasis, rheumatoid arthritis, and multiple sclerosis, largely through its role in recruiting CCR6⁺ Th17 cells. CCL20 also contributes to tumor microenvironment modulation in certain cancers by influencing immune cell infiltration and inflammation. As a central mediator of mucosal immunity and Th17-associated inflammation, human CCL20 is widely studied as a biomarker of inflammatory activation and a potential therapeutic target in autoimmune disease, infection, and cancer.
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