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Ovine CCL3 (MIP-1 alpha) Recombinant Protein
- Catalog Number:
- RP1529V
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Budorcas taxicolor (takin), Ovis aries (sheep)
- Ovine CCL3 (MIP-1α, Macrophage Inflammatory Protein-1 alpha) (catalog RP1529V) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~7.8 kDa, 70 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to sheep and takin. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study CCL3/MIP-1α signaling and immune-cell chemotaxis (including recruitment and activation of monocytes, T cells, and other leukocytes during inflammatory responses); typical experimental uses include cell-culture stimulation and migration assays, chemotaxis and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | APFGADTPTA CCFSYAARQL SRKIVADYFE TSSQCSKPGV IFQTKRGRQV CANPSEDWVQ QYVTDLELNS (70) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 7.8 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Ovine CCL3 (C-C motif chemokine ligand 3, also known as MIP-1α, macrophage inflammatory protein-1 alpha) is a pro-inflammatory chemokine that regulates recruitment and activation of immune cells during inflammatory and infectious responses in sheep (Ovis aries). CCL3 primarily signals through the CCR1 and CCR5 receptors, promoting chemotaxis of monocytes, macrophages, dendritic cells, natural killer (NK) cells, and activated T lymphocytes to sites of infection or tissue injury. In sheep, CCL3 is produced by activated macrophages, dendritic cells, epithelial cells, and lymphocytes in response to inflammatory stimuli such as microbial components and cytokines including TNF-α and IL-1β. CCL3-mediated immune cell recruitment plays an important role in host defense against pathogens associated with respiratory infections, mastitis, and parasitic diseases common in small ruminants, where infiltration of monocytes and lymphocytes contributes to pathogen clearance and immune activation. While CCL3-driven leukocyte trafficking supports protective immune responses and tissue repair, excessive or prolonged expression may contribute to chronic inflammation and tissue damage. As a regulator of immune cell migration and inflammatory signaling, ovine CCL3 is important for studies of host-pathogen interactions, inflammatory disease mechanisms, and immune responses relevant to sheep health and small ruminant veterinary research.
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