Dolphin CCL5 (RANTES) Recombinant Protein

Catalog Number:
RP1532P
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Tursiops truncatus (bottlenose dolphin)
  • Dolphin CCL5 (RANTES, Regulated upon Activation, Normal T Cell Expressed and Secreted) (catalog RP1532P) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~7.9 kDa, 68 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to bottlenose dolphin. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study CCL5/RANTES signaling and immune-cell chemotaxis (including recruitment of T cells, eosinophils, and other leukocytes during inflammatory responses); typical experimental uses include cell-culture stimulation and migration assays, chemotaxis and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceSPYASDTTPC CFAYLSRPLP RAHLREYFYT SSKCSMAAVV FITRKNRQVC ANPEKKWVKE YINTLELS (68)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight7.9 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Dolphin CCL5 (C–C motif chemokine ligand 5, also known as RANTES – Regulated upon Activation, Normal T cell Expressed and Secreted) is a proinflammatory chemokine that regulates immune cell recruitment and inflammatory responses in dolphins, particularly the bottlenose dolphin (Tursiops truncatus). CCL5 is a member of the CC chemokine family, which also includes CCL3 (MIP-1α), CCL4 (MIP-1β), and CCL2 (MCP-1), chemokines that coordinate leukocyte trafficking during immune activation. In dolphins, CCL5 is produced primarily by activated T lymphocytes, macrophages, dendritic cells, platelets, and epithelial cells in response to infection, inflammatory stimuli, or tissue injury, and exerts its biological effects mainly through binding to the chemokine receptors CCR1, CCR3, and CCR5, promoting chemotaxis and activation of T cells, monocytes/macrophages, eosinophils, and natural killer (NK) cells at sites of inflammation. Through these actions, dolphin CCL5 contributes to immune defense against viral, bacterial, and parasitic pathogens encountered in marine environments. Because dolphins are considered sentinel species for ocean health, studying chemokines such as CCL5 helps improve understanding of marine mammal immunology, host responses to infectious disease, and the effects of environmental stressors or pollutants on immune function.

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