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Swine CTLA-4 Recombinant Protein
- Catalog Number:
- RP1534S
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Sus scrofa (pig)
- Swine CTLA-4 (Cytotoxic T-Lymphocyte-Associated Protein 4, CD152) (catalog RP1534S) is a yeast-derived immune checkpoint protein supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~13.3 kDa, 124 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to pig. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study CTLA-4 signaling and immune regulation (including inhibition of T-cell activation and modulation of immune checkpoint pathways); typical experimental uses include cell-culture stimulation and binding assays, immune checkpoint and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | MHVAQPAVVL ANSRGVASFV CEYGSAGKAA EVRVTVLRRA GSQMTEVCAA TYTVEDELTF LDDSTCTGTS TENKVNLTIQ GLRAVDTGLY ICKVELLYPP PYYVGMGNGT QIYVIDPEPC PDSD (124) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 13.3 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Swine CTLA-4 (Cytotoxic T-Lymphocyte-Associated Protein 4, also known as CD152) is an immune checkpoint receptor belonging to the immunoglobulin superfamily that functions as a key negative regulator of T-cell activation in pigs (Sus scrofa). CTLA-4 is primarily expressed on activated CD4⁺ and CD8⁺ T lymphocytes and on regulatory T cells (Tregs) following antigen stimulation. It binds to the costimulatory ligands CD80 (B7-1) and CD86 (B7-2) on antigen-presenting cells such as dendritic cells, macrophages, and B cells, competing with the activating receptor CD28 for these ligands. Engagement of CTLA-4 transmits inhibitory signals that suppress T-cell proliferation, cytokine production, and immune activation, thereby helping maintain immune homeostasis and peripheral tolerance. In swine, CTLA-4 signaling is particularly relevant during viral infections such as porcine reproductive and respiratory syndrome virus (PRRSV), where excessive or dysregulated T-cell responses can influence disease outcome. Pigs are also used as large-animal models for transplantation immunology, and CTLA-4-Ig fusion proteins (similar to the human therapeutic abatacept/belatacept) have been tested in pig-to-nonhuman primate xenotransplantation studies, where blockade of CD28-mediated costimulation through the CTLA-4 pathway helps reduce T-cell-mediated graft rejection. These studies make swine CTLA-4 an important component of translational research on immune checkpoint regulation and tolerance induction in transplantation and immunotherapy.
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