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Swine Osteopontin (OPN) Recombinant Protein
- Catalog Number:
- RP1546S
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Sus scrofa (pig)
- Swine OPN (Osteopontin) (catalog RP1546S) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~31.9 kDa, 287 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to pig. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study osteopontin signaling and cellular processes (including regulation of cell adhesion, migration, immune responses, and bone remodeling); typical experimental uses include cell-culture stimulation and adhesion/migration assays, signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | LPVKQTNSGS SEEKLLSNKY TDAVATLLKP DPSQKQTFLA PQNTISSEET DDFKQETLPS KSNESPEQTD DVDDDDDEDH VDSRDTDSEE ADHADDADRS DESHHSDESD ELVTDFPTDT PATDVTPAVP TGDPNDGRGD SVVYGLRSKS KKFRRSEAQQ LDATEEDLTS HVESEETDGT PKAILVAQRL HVASDLDSQE KDSQETSQPD DRSVETRSQE QSKEYTIKTY DGSNEHSNVI ESQENPKVSQ EFHSHEDKLV PDSKSEEDKH LKLRVSHELE SASSEIN (287) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 31.9 kda |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Swine Osteopontin (OPN) (also known as SPP1, Secreted Phosphoprotein 1, bone sialoprotein I, ETA-1, or early T-lymphocyte activation-1) is a multifunctional phosphorylated glycoprotein involved in immune regulation, cell adhesion, and tissue remodeling in pigs (Sus scrofa domesticus). Osteopontin interacts with integrins (such as αvβ3, αvβ1, and α4β1) and CD44 receptors, activating signaling pathways that regulate cell migration, inflammatory responses, and extracellular matrix remodeling. In pigs, OPN is produced by osteoblasts, macrophages, epithelial cells, and activated T lymphocytes, and plays roles in bone metabolism, immune responses, and tissue repair. In swine health, osteopontin is particularly relevant in reproductive biology, where it is expressed in the uterine endometrium and conceptus during early pregnancy, contributing to embryo implantation, placental attachment, and maternal-fetal communication. OPN is also involved in immune responses during respiratory and enteric infections, including conditions such as porcine reproductive and respiratory syndrome virus (PRRSV) and bacterial infections, where it can influence leukocyte recruitment and inflammatory signaling. While osteopontin supports immune defense and tissue repair, excessive expression may contribute to chronic inflammation or fibrotic tissue remodeling. Because pigs are widely used as large-animal models for human physiology, immunology, and reproductive biology, characterization of swine osteopontin supports studies of immune regulation, implantation biology, tissue remodeling, and host-pathogen interactions relevant to both veterinary medicine and translational biomedical research.
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