Chicken SCF (Stem Cell Factor) Recombinant Protein

Catalog Number:
RP1555C
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Gallus gallus (chicken)
  • Chicken SCF (Stem Cell Factor, KIT Ligand, KITLG) (catalog RP1555C) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~22.5 kDa, 200 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to chicken. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study SCF/KIT signaling and hematopoiesis (including regulation of stem cell maintenance, proliferation, and differentiation); typical experimental uses include cell-culture stimulation and differentiation assays, cytokine signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceQSSCGNPVTD DVNDIAKLVG NLPNDYLITL KYVPKMDSLP NHCWLHLMVP EFSRSLHNLL QKFSDISDMS DVLSNYSIIN NLTRIINDLM ACLAFDKNKD FIKENGHLYE EDRFIPENFF RLFNSTIEVY KEFADSLDKN DCIMPSTVET PENDSRVAVT KTISFPPVAA SSLRNDSIGS NTSSNSNKEA LGFISSSSLQ (200)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight22.5 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Chicken Stem Cell Factor (SCF) (also known as KIT ligand, KITLG, mast cell growth factor, or steel factor) is a cytokine and growth factor involved in hematopoiesis, stem cell maintenance, and immune cell development in chickens (Gallus gallus). SCF belongs to a group of hematopoietic growth factors that regulate the survival, proliferation, and differentiation of stem and progenitor cells. It functions by binding to its receptor c-Kit (CD117), a receptor tyrosine kinase expressed on hematopoietic stem cells, mast cells, melanocyte precursors, and germ cells, triggering intracellular signaling pathways including PI3K/AKT, MAPK, and JAK/STAT that promote cell survival and growth. In chickens, SCF is produced by stromal cells, fibroblasts, endothelial cells, and epithelial cells, and can exist in both membrane-bound and soluble forms, enabling both localized and systemic regulation of c-Kit–expressing cells. Chicken SCF contributes to bone marrow and thymic hematopoiesis, immune cell development, pigmentation, and germ cell development during embryogenesis and growth. Because chickens are important models for avian immunology, developmental biology, and hematopoietic research, characterization of chicken SCF supports studies of stem cell regulation, immune system development, and conserved growth factor signaling pathways relevant to both poultry health and vertebrate biology.

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1000 Westgate Drive
Suite 123
Saint Paul, MN 55114

Phone: 651-646-0089
Fax: 651-646-0095

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