Zebrafish CCL2 (MCP-1) Recombinant Protein

Catalog Number:
RP1575Z
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Danio rerio (zebrafish)
  • Zebrafish CCL2 (MCP-1, Monocyte Chemoattractant Protein-1) (catalog RP1575Z) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~14.3 kDa, 136 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to zebrafish. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study CCL2/MCP-1 signaling and immune-cell chemotaxis (including recruitment of monocytes and other leukocytes during inflammatory responses in zebrafish models); typical experimental uses include cell-culture stimulation and migration assays, chemotaxis and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequencePIPINSAKSV CCEATTHRNI PLKQIMSYQW TTSTCPIKAI VFKTIAGREI CVDPQNTLVK KQVAKLDKRR STTALSPKST STTAETPAAT SGTTSSSEST SAKSSPASTS ATALSHEYTS ASTVTFTTAE SHSTSV (136)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight14.3 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Zebrafish (Danio rerio) CCL2 (C-C motif chemokine ligand 2, also referred to as an MCP-1–like chemokine) is a pro-inflammatory chemokine involved in recruitment and activation of monocytes/macrophages and other leukocytes during innate immune responses. Similar to its mammalian counterpart, zebrafish CCL2-like signaling promotes leukocyte migration to sites of infection, tissue injury, or inflammation through chemokine receptor pathways functionally related to CCR2, coordinating early immune responses and inflammatory cytokine production. In zebrafish, CCL2 expression is induced during bacterial infections (e.g., Mycobacterium marinum), viral challenges, and tissue damage, where macrophage recruitment is essential for pathogen containment, granuloma formation, and tissue repair. Zebrafish models have been widely used to visualize chemokine-driven immune cell migration in vivo using fluorescent reporter lines and live imaging, allowing detailed study of macrophage dynamics and inflammatory signaling. While CCL2-mediated leukocyte recruitment supports host defense and wound healing, excessive or sustained expression can contribute to chronic inflammation or tissue pathology. Because zebrafish are a powerful vertebrate model for innate immunity, infection biology, and inflammatory disease, characterization of zebrafish CCL2-like chemokine signaling provides important insight into the evolution of chemokine networks and mechanisms of immune cell trafficking relevant to human inflammatory and infectious diseases.

Please choose a size to see price


Reagents for Animal Model and Animal Health Research

Kingfisher Biotech, Inc.
1000 Westgate Drive
Suite 123
Saint Paul, MN 55114

Phone: 651-646-0089
Fax: 651-646-0095

Animal Antibodies

Newsletter

"