Cynomolgus Monkey CCL3 (MIP-1 alpha) Recombinant Protein

Catalog Number:
RP1596Y
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Macaca fascicularis (crab-eating macaque), Macaca mulatta (Rhesus monkey), Macaca nemestrina (pig-tailed macaque)
  • Cynomolgus Monkey CCL3 (MIP-1α, Macrophage Inflammatory Protein-1 alpha) (catalog RP1596Y) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~8.3 kDa, 74 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to crab-eating macaque, rhesus monkey, and pig-tailed macaque. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study CCL3/MIP-1α signaling and immune-cell chemotaxis (including recruitment and activation of monocytes, T cells, and other leukocytes during inflammatory responses); typical experimental uses include cell-culture stimulation and migration assays, chemotaxis and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceNRISATFAAD TPTSCCFSYI SRQIPQNFIA DYFETNSQCS KPGVIFLTKR GRQVCADPSK EWVQKYVSDL ELSA (74)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight8.3 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Cynomolgus Monkey (Macaca fascicularis) CCL3 (C-C motif chemokine ligand 3, also known as MIP-1α, macrophage inflammatory protein-1 alpha) is a pro-inflammatory chemokine that plays an important role in recruitment and activation of immune cells during inflammatory and infectious responses in nonhuman primates. CCL3 primarily signals through the CCR1 and CCR5 receptors, promoting chemotaxis of monocytes, macrophages, dendritic cells, natural killer (NK) cells, and activated T lymphocytes to sites of infection or tissue injury. In cynomolgus monkeys, CCL3 is produced by activated macrophages, dendritic cells, epithelial cells, and lymphocytes in response to inflammatory stimuli such as pathogen-associated molecules and cytokines including TNF-α and IL-1β. Because cynomolgus monkeys are widely used in preclinical infectious disease and immunology research, CCL3 is studied in models of viral infections (such as dengue, influenza, and SARS-CoV-2), tuberculosis, and inflammatory diseases, where recruitment of monocytes and T cells contributes to immune activation and pathogen control. CCL3-mediated signaling also influences immune cell infiltration and inflammatory responses in tissues during disease progression. As a regulator of leukocyte trafficking and inflammatory signaling, cynomolgus monkey CCL3 is important for studies of host–pathogen interactions, immune regulation, and evaluation of immunomodulatory therapeutics, providing translational insight into chemokine-driven immune responses relevant to human disease.

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