Cynomolgus Monkey IL-1 beta Recombinant Protein

Catalog Number:
RP1597Y
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Macaca fascicularis (crab-eating macaque), Macaca mulatta (Rhesus monkey)
  • Cynomolgus Monkey IL-1β (Interleukin-1 beta) (catalog RP1597Y) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~17.2 kDa, 152 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to crab-eating macaque and rhesus monkey. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study IL-1β signaling and inflammatory responses (including regulation of immune activation, cytokine production, and fever/inflammation pathways); typical experimental uses include cell-culture stimulation and cytokine assays, signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceAPVRSLHCTL RDAQLKSLVM SGPYELKALH LQGQDLEQQV VFSMSFVQGE ESNDKIPVAL GLKAKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQFPNW YISTSQAENM PVFLGGTRGG QDITDFTMQF VS (152)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight17.2 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Cynomolgus Monkey (Macaca fascicularis) Interleukin-1 Beta (IL-1β) is a potent pro-inflammatory cytokine produced primarily by activated macrophages, monocytes, and dendritic cells in response to infection, tissue injury, or inflammatory stimuli. Synthesized as an inactive precursor (pro–IL-1β), it is cleaved into its active form by caspase-1 within inflammasome complexes—most notably the NLRP3 inflammasome—and signals through the IL-1 receptor type 1 (IL-1R1), activating NF-κB and MAPK pathways that drive fever, leukocyte recruitment, endothelial activation, and acute-phase responses. Highly homologous to human IL-1β, cynomolgus IL-1β plays a central role in nonhuman primate models of infectious diseases such as dengue, influenza, SARS-CoV-2, tuberculosis, and bacterial sepsis, where its expression correlates with systemic inflammation and disease severity. It is also relevant in models of autoimmune disease, vaccine-induced immune activation, and immunotoxicology studies. Because cynomolgus monkeys are widely used in preclinical safety assessment and translational biomedical research, characterization of IL-1β is essential for evaluating inflammasome activation, cytokine release syndromes, and therapeutic modulation of inflammatory pathways, bridging mechanistic insights between nonhuman primate models and human inflammatory disease.

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