Multi-species (Human, Monkey) Midkine Recombinant Protein

Catalog Number:
RP1598HY
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Chlorocebus sabaeus (green monkey), Gorilla gorilla gorilla (western lowland gorilla), Homo sapiens (human), Macaca fascicularis (crab-eating macaque), Macaca mulatta (Rhesus monkey), Mandrillus leucophaeus (drill), Papio anubis (olive baboon), Pongo abelii (Sumatran orangutan), Rhinopithecus bieti (black snub-nosed monkey), Rhinopithecus roxellana (golden snub-nosed monkey), Theropithecus gelada (gelada)
  • Human/Cynomolgus Monkey Midkine (MK) (catalog RP1598HY) is a yeast-derived growth factor supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~13.4 kDa, 123 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to human, crab-eating macaque, western lowland gorilla, green monkey, rhesus monkey, Sumatran orangutan, drill, olive baboon, black snub-nosed monkey, golden snub-nosed monkey, and gelada. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study midkine signaling and cellular processes (including regulation of cell growth, survival, migration, and development); typical experimental uses include cell-culture stimulation and proliferation assays, signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceVAKKKDKVKK GGPGSECAEW AWGPCTPSSK DCGVGFREGT CGAQTQRIRC RVPCNWKKEF GADCKYKFEN WGACDGGTGT KVRQGTLKKA RYNAQCQETI RVTKPCTPKT KAKAKAKKGK GKD (123)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight13.4 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Human/Cynomolgus Monkey Midkine (MK) is a heparin-binding growth factor and cytokine that plays important roles in cell growth, survival, and tissue repair in humans (Homo sapiens) and cynomolgus monkeys (Macaca fascicularis). The midkine protein is identical at the amino acid level in these two species, reflecting strong evolutionary conservation and its essential biological functions in primates. Midkine is highly expressed during embryonic development and can also be induced in adult tissues in response to injury, inflammation, or physiological stress. Human and cynomolgus monkey midkine interact with several cell surface receptors, including receptor-type protein tyrosine phosphatase ζ (PTPRZ1), low-density lipoprotein receptor–related proteins (LRPs), and integrins, activating intracellular signaling pathways such as MAPK, PI3K–AKT, and NF-κB that regulate cell proliferation, migration, survival, and angiogenesis. Through these mechanisms, midkine contributes to processes including tissue regeneration, immune cell recruitment, neural development, and inflammatory responses. In cell culture, recombinant human/cynomolgus monkey midkine is commonly used to stimulate cell proliferation and migration and to study growth factor–mediated signaling pathways involved in development, inflammation, and tissue repair. Because cynomolgus monkeys are widely used as nonhuman primate research models, midkine is also studied in translational biomedical research to investigate mechanisms of development, tissue injury, and disease relevant to human health.

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