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Feline CCL3 (MIP-1 alpha) Recombinant Protein
- Catalog Number:
- RP1603F
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Acinonyx jubatus (cheetah), Felis catus (domestic cat), Panthera pardus (leopard), Panthera tigris altaica (Amur tiger), Puma concolor (puma)
- Feline CCL3 (MIP-1α, Macrophage Inflammatory Protein-1 alpha) (catalog RP1603F) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~7.8 kDa, 70 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to domestic cat, cheetah, leopard, Amur tiger, and puma. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study CCL3/MIP-1α signaling and immune-cell chemotaxis (including recruitment and activation of monocytes, T cells, and other leukocytes during inflammatory responses); typical experimental uses include cell-culture stimulation and migration assays, chemotaxis and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | APFGADTPTA CCFSYVSKQI PRKFIADYFE TSSQCSKPGV IFQTRRGRQV CADPSEAWVQ EYVTDLELSA (70) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 7.8 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Feline CCL3 (C-C motif chemokine ligand 3, also known as MIP-1α, macrophage inflammatory protein-1 alpha) is a pro-inflammatory chemokine that regulates recruitment and activation of immune cells during inflammatory and infectious responses in cats (Felis catus). CCL3 primarily signals through the CCR1 and CCR5 receptors, promoting chemotaxis of monocytes, macrophages, natural killer (NK) cells, dendritic cells, and activated T lymphocytes to sites of infection or tissue injury. In cats, CCL3 is produced by activated macrophages, dendritic cells, epithelial cells, and lymphocytes in response to inflammatory stimuli such as microbial products or cytokines including TNF-α and IL-1β. CCL3-mediated signaling plays an important role in feline immune responses to viral infections such as feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV), as well as inflammatory conditions including feline infectious peritonitis (FIP) caused by feline coronavirus, where recruitment of macrophages and T cells contributes to immune defense and inflammatory pathology. While CCL3-driven leukocyte recruitment supports pathogen clearance and immune activation, excessive or persistent expression may contribute to chronic inflammation and tissue damage. As a biomarker of inflammatory immune cell recruitment, feline CCL3 is studied in investigations of host-pathogen interactions, immune regulation, and disease pathogenesis relevant to feline health and comparative immunology.
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