Equine Amphiregulin Recombinant Protein

Catalog Number:: RP1605E
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Equus asinus (ass), Equus caballus (horse), Equus przewalskii (Przewalski's horse)

Equine Amphiregulin (AR) (catalog RP1605E) is a yeast-derived growth factor supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~10.1 kDa, 87 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with amino-acid homology to horse, Przewalski's horse, and ass. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study amphiregulin/EGFR signaling and cellular processes (including regulation of cell proliferation, differentiation, and tissue repair); typical experimental uses include cell-culture stimulation and growth assays, signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.

Amino Acid Sequence	SIRVEQVVKP KKNKTESEKT SDKPKRKKKG GKNGKNRRNK KKKNPCDAEF QNFCIHGECK YIEHLEAVTC QCHQDYFGER CGEKSMK (87)
Endotoxin	Naturally endotoxin-free

Storage Conditions	-20°C
Molecular Weight	10.1 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Equine amphiregulin (AREG) is a member of the epidermal growth factor (EGF) family and functions as a ligand for the epidermal growth factor receptor (EGFR/ErbB1), where it regulates epithelial cell proliferation, differentiation, survival, and tissue repair. In horses (Equus caballus), amphiregulin is produced by epithelial cells, keratinocytes, fibroblasts, airway smooth muscle cells, and activated immune cells in response to tissue injury, inflammatory cytokines, and allergic stimulation. Through activation of EGFR-dependent signaling pathways such as MAPK and PI3K/AKT, equine amphiregulin contributes to wound healing, mucosal barrier maintenance, and tissue remodeling in cutaneous, respiratory, and gastrointestinal tissues. Dysregulated expression may be associated with chronic inflammation, fibrosis, or tumor development. In veterinary and comparative medicine research, equine amphiregulin is of particular interest in models of equine asthma (inflammatory airway disease), recurrent airway obstruction, chronic wounds (including distal limb wound healing), laminitis-associated tissue remodeling, and neoplastic conditions such as squamous cell carcinoma and sarcoids where EGFR signaling may play a role. Characterizing amphiregulin expression in horses provides valuable insight into epithelial-immune interactions, tissue repair mechanisms, and EGFR-driven inflammatory or proliferative processes in equine health and translational research.

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