Multi-species (Human, Monkey) VEGF-A (121aa) Recombinant Protein

Catalog Number:
RP1610H
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Cercocebus atys (sooty mangabey), Chlorocebus sabaeus (green monkey), Colobus angolensis palliates (Peter’s Angolan Colobus), Gorilla gorilla gorilla (western lowland gorilla), Homo sapiens (human), Macaca fascicularis (crab-eating macaque), Macaca mulatta (Rhesus monkey), Macaca nemestrina (pig-tailed macaque), Mandrillus leucophaeus (drill), Nomascus leucogenys (northern white-cheeked gibbon), Pan paniscus (pygmy chimpanzee), Pan troglodytes (chimpanzee), Papio anubis (olive baboon), Rhinopithecus roxellana (golden snub-nosed monkey)
  • Human/Cynomolgus Monkey VEGF-A (Vascular Endothelial Growth Factor A, 121AA) (catalog RP1610H) is a yeast-derived growth factor supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~14.1 kDa, 121 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to human, crab-eating macaque, sooty mangabey, green monkey, Peter's Angolan colobus, western lowland gorilla, rhesus monkey, pig-tailed macaque, drill, northern white-cheeked gibbon, pygmy chimpanzee, chimpanzee, olive baboon, and golden snub-nosed monkey. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study VEGF-A signaling and angiogenesis (including regulation of endothelial cell proliferation, migration, vascular permeability, and blood vessel formation); typical experimental uses include cell-culture stimulation and angiogenesis assays, signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceAPMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQEKCDKPR R (121)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight14.1 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Human and Cynomolgus Monkey Vascular Endothelial Growth Factor A (VEGF-A) is a member of the vascular endothelial growth factor (VEGF) family, which includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, and placental growth factor (PlGF)—key regulators of angiogenesis, vascular permeability, and endothelial cell growth. In both humans (Homo sapiens) and cynomolgus monkeys (Macaca fascicularis), the VEGF-A protein is identical at the amino acid level, reflecting strong evolutionary conservation and similar biological functions in these primate species. VEGF-A is produced by many cell types including endothelial cells, macrophages, fibroblasts, epithelial cells, smooth muscle cells, and tumor cells, particularly in response to hypoxia, inflammation, and tissue injury. VEGF-A exerts its biological effects primarily through binding to VEGF receptor-1 (VEGFR-1/Flt-1) and VEGF receptor-2 (VEGFR-2/KDR) on endothelial cells, activating intracellular signaling pathways such as MAPK/ERK, PI3K/AKT, and PLCγ, which promote endothelial cell proliferation, migration, vascular permeability, and new blood vessel formation. In primates, VEGF-A plays essential roles in embryonic vascular development, wound healing, tissue regeneration, and reproductive physiology, including placental vascularization during pregnancy. Dysregulated VEGF-A expression is strongly associated with tumor angiogenesis, diabetic retinopathy, age-related macular degeneration, and other vascular diseases. Because cynomolgus monkeys are widely used as preclinical models for human vascular biology and therapeutic development, the identical VEGF-A sequence between these species supports their use in translational studies of angiogenesis and evaluation of anti-VEGF therapies such as monoclonal antibodies and receptor inhibitors used in cancer and ophthalmic diseases.

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