Human M-CSF Recombinant Protein

Catalog Number:: RP1614H
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Chlorocebus sabaeus (green monkey), Gorilla gorilla gorilla (western lowland gorilla), Homo sapiens (human), Macaca mulatta (Rhesus monkey), Papio anubis (olive baboon), Piliocolobus tephrosceles (Ugandan red Colobus), Rhinopithecus roxellana (golden snub-nosed monkey), Theropithecus gelada (gelada)

Human M-CSF (Macrophage Colony-Stimulating Factor, CSF-1) (catalog RP1614H) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~18.4 kDa, 158 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to human M-CSF, western lowland gorilla, green monkey, rhesus monkey, olive baboon, Ugandan red colobus, golden snub-nosed monkey, and gelada. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study M-CSF signaling and hematopoiesis (including regulation of macrophage differentiation, proliferation, and survival); typical experimental uses include cell-culture stimulation and differentiation assays, cytokine signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.

Amino Acid Sequence	EEVSEYCSHM IGSGHLQSLQ RLIDSQMETS CQITFEFVDQ EQLKDPVCYL KKAFLLVQDI MEDTMRFRDN TPNAIAIVQL QELSLRLKSC FTKDYEEHDK ACVRTFYETP LQLLEKVKNV FNETKNLLDK DWNIFSKNCN NSFAECSSQG HERQSEGS (158)
Endotoxin	Naturally endotoxin-free

Storage Conditions	-20°C
Molecular Weight	18.4 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Human Macrophage Colony-Stimulating Factor (M-CSF) is a cytokine that regulates the development, proliferation, and survival of monocytes and macrophages in humans (Homo sapiens). M-CSF is produced by several cell types, including fibroblasts, endothelial cells, epithelial cells, and stromal cells, in response to tissue signals and inflammatory stimuli. Human M-CSF binds to the colony-stimulating factor-1 receptor (CSF1R), a receptor tyrosine kinase expressed on monocytes and macrophage lineage cells, activating intracellular signaling pathways such as PI3K-AKT, ERK/MAPK, and JAK-STAT that promote differentiation and maintenance of macrophage populations. Through these mechanisms, M-CSF supports macrophage-mediated immune surveillance, phagocytosis, tissue homeostasis, and inflammatory responses. Because macrophages play a central role in host defense and immune regulation, M-CSF-mediated macrophage function is important for maintaining immune health and responding to infection or tissue injury. In cell culture, recombinant human M-CSF is commonly used to promote differentiation of human monocytes into macrophages and to support macrophage proliferation and functional assays in vitro. Human M-CSF is also widely studied in biomedical research, including investigations of macrophage biology, inflammation, cancer, and immune-mediated diseases.

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