Dolphin CCL4 (MIP-1 beta) Recombinant Protein

Catalog Number:
RP1615P
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Tursiops truncatus (bottlenose dolphin)
  • Dolphin CCL4 (MIP-1β, Macrophage Inflammatory Protein-1 beta) (catalog RP1615P) is a yeast-derived chemokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~8.4 kDa, 73 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to bottlenose dolphin. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study CCL4/MIP-1β signaling and immune-cell chemotaxis (including recruitment and activation of T cells, monocytes, and other leukocytes during inflammatory responses); typical experimental uses include cell-culture stimulation and migration assays, chemotaxis and signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceLEKRAPMSSD PPTACCFTYT LRKLPRHFVI DYYETSSLCS QPAVVFQTKK GRQVCANPSD PWVQEYMDDL ELN (73)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight8.4 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Dolphin CCL4 (C-C motif chemokine ligand 4, also known as MIP-1β, macrophage inflammatory protein-1 beta) is a pro-inflammatory chemokine involved in recruitment and activation of immune cells during inflammatory and antiviral immune responses in dolphins, particularly the bottlenose dolphin (Tursiops truncatus). CCL4 primarily signals through the CCR5 receptor, promoting chemotaxis of monocytes, macrophages, dendritic cells, natural killer (NK) cells, and activated T lymphocytes to sites of infection or tissue injury. In dolphins, CCL4 is likely produced by activated macrophages, dendritic cells, epithelial cells, and lymphocytes in response to inflammatory stimuli such as microbial components and cytokines including TNF-α and IL-1β. CCL4-mediated immune cell recruitment may play important roles in responses to respiratory infections, skin lesions, and wound healing, as well as in immune defense against viral and bacterial pathogens affecting marine mammals. Because dolphins are frequently exposed to environmental stressors such as pollutants, harmful algal blooms, and emerging infectious diseases, chemokines like CCL4 may also contribute to regulation of inflammatory responses associated with environmental immunotoxicology. As dolphins serve as sentinel species for marine ecosystem health, characterization of dolphin CCL4 supports research into immune regulation, host–pathogen interactions, and the impacts of environmental stress on marine mammal immune function.

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