Feline IFN alpha Recombinant Protein

Catalog Number:: RP1629F
Application:: Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:: Felis catus (domestic cat)

Product Description

Feline IFN-α (Interferon alpha) (catalog RP1629F) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~19.7 kDa, 171 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to domestic cat. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study IFN-α signaling and antiviral immune responses (including regulation of innate immunity, antiviral defense, and immune modulation); typical experimental uses include cell-culture stimulation and antiviral assays, cytokine signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.

Properties/Specifications

Amino Acid Sequence	CDLPQTHGLL NRRALTLLGQ MRRLPASSCQ KDRNDFAFPQ DVFGGDQSHK AQALSVVHVT NQKIFHFFCT EASSSAAWNT TLLEEFCTGL DRQLTRLEAC VLQEVEEGEA PLTNEDIHPE DSILRNYFQR LSLYLQEKKY SPCAWEIVRA EIMRSLYYSS TALQKRLRSE K (171)
Endotoxin	Naturally endotoxin-free
Form	Lyophilized
Storage Conditions	-20°C
Molecular Weight	19.7 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Affinity Tag	None
aminoAcids	171
Carrier Protein	None
Expression System	Yeast
Form	Lyophilized
Country Of Origin	USA

Feline Interferon-Alpha (IFN-α) is a type I interferon produced primarily by plasmacytoid dendritic cells and virus-infected cells in cats (Felis catus), where it plays a central role in innate antiviral immunity by inducing interferon-stimulated genes (ISGs), enhancing antigen presentation, and activating natural killer (NK) cells and adaptive immune responses through the IFNAR receptor complex and JAK/STAT signaling pathways. In feline health, IFN-α is critically involved in host defense against viral infections such as feline leukemia virus (FeLV), feline immunodeficiency virus (FIV), feline infectious peritonitis (FIP; caused by feline coronavirus), feline herpesvirus, and calicivirus, and recombinant feline IFN-α has been used therapeutically to modulate antiviral and immune responses. Dysregulated or insufficient IFN-α responses can contribute to viral persistence, immunopathology, or chronic inflammation. Importantly, cats serve as valuable natural animal models for human retroviral infections (FIV as a model for HIV), coronavirus-associated immune dysregulation (FIP as a model for severe systemic coronavirus disease), and certain oncogenic viral conditions, making characterization of feline IFN-α highly relevant for understanding antiviral immunity, viral immune evasion, and interferon-based therapeutic strategies in both veterinary and comparative biomedical research.