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Ovine IL-1 alpha Recombinant Protein
- Catalog Number:
- RP1630V
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Ovis aries (sheep)
- Ovine IL-1α (Interleukin-1 alpha) (catalog RP1630V) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~17.9 kDa, 156 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to sheep. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study IL-1α signaling and inflammatory responses (including regulation of immune activation, cytokine production, and inflammatory pathways); typical experimental uses include cell-culture stimulation and cytokine assays, signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | SAHYSFQSNV KYNFMRVIHQ ECILNDALNQ SIIRDMSGPY LTAATLNNLE EAVKFDMVAY VSEEDSQLPV TLRISKTQLF VSAQNEDEPV LLKEMPETPK IIKDETNLLF FWEKHGSMDY FKSVAHPKLF IATKQEKLVH MASGPPSITD FQILEK (156) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 17.9 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Ovine Interleukin-1 Alpha (IL-1α) is a pro-inflammatory cytokine belonging to the IL-1 family-which includes IL-1α, IL-1β, and the IL-1 receptor antagonist (IL-1Ra)-and plays a central role in initiating innate and sterile inflammatory responses in sheep (Ovis aries). IL-1α is constitutively expressed in epithelial and barrier tissues and functions as an intracellular alarmin that is rapidly released upon cell injury, necrosis, microbial invasion, or mechanical stress, triggering early inflammatory signaling independent of inflammasome processing. Upon binding to the IL-1 receptor type 1 (IL-1R1), ovine IL-1α activates NF-κB and MAPK pathways that promote leukocyte recruitment, endothelial activation, fever, and amplification of downstream pro-inflammatory cytokine cascades. In ovine health, IL-1α is particularly relevant in inflammatory conditions such as mastitis, pneumonia, foot rot, enteric infections, parasitic infestations, and viral diseases including ovine progressive pneumonia (maedi-visna), where epithelial and tissue damage drive early IL-1α-mediated immune activation. While essential for pathogen defense and tissue repair, excessive IL-1α signaling can contribute to tissue damage, chronic inflammation, and reduced productivity. As an early mediator of sterile and infectious inflammation, ovine IL-1α serves as a biomarker of tissue injury and innate immune activation. In veterinary and comparative research, characterization of ovine IL-1α supports studies of host-pathogen interactions, inflammatory regulation in small ruminants, and development of targeted IL-1-modulating therapies to improve flock health and disease resilience.
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