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Rat IL-1 alpha Recombinant Protein
- Catalog Number:
- RP1631R
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Rattus norvegicus (Norway rat)
- Rat IL-1α (Interleukin-1 alpha) (catalog RP1631R) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~17.8 kDa, 156 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to Norway rat. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study IL-1α signaling and inflammatory responses (including regulation of immune activation, cytokine production, and inflammatory pathways); typical experimental uses include cell-culture stimulation and cytokine assays, signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | SAPHSFQNNL RYKLIRIVKQ EFIMNDSLNQ NIYVDMDRIH LKAASLNDLQ LEVKFDMYAY SSGGDDSKYP VTLKVSNTQL FVSAQGEDKP VLLKEIPETP KLITGSETDL IFFWEKINSK NYFTSAAFPE LLIATKEQSQ VHLARGLPSM IDFQIS (156) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 17.8 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Rat Interleukin-1 Alpha (IL-1α) is a pro-inflammatory cytokine belonging to the IL-1 family-which includes IL-1α, IL-1β, and the IL-1 receptor antagonist (IL-1Ra)-and plays a central role in initiating innate and sterile inflammatory responses in rats (Rattus norvegicus). IL-1α is constitutively expressed in epithelial, endothelial, and barrier tissues and functions as an intracellular alarmin that is rapidly released upon cell injury, necrosis, ischemia, or toxic insult, triggering early inflammatory signaling independent of inflammasome processing. Upon binding to the IL-1 receptor type 1 (IL-1R1), rat IL-1α activates NF-κB and MAPK pathways that promote leukocyte recruitment, endothelial activation, fever, and amplification of downstream pro-inflammatory cytokine cascades. In rat models, IL-1α is widely studied in experimental systems of stroke and ischemia-reperfusion injury, traumatic brain injury, arthritis, colitis, neuropathic pain, and cancer-associated inflammation, where it contributes to early tissue inflammation and immune cell infiltration. Because rats are frequently used in pharmacology, toxicology, neuroscience, and cardiovascular research, characterization of IL-1α is critical for understanding sterile inflammation, tissue injury responses, and cytokine-driven pathology. As both a biomarker and therapeutic target, rat IL-1α supports preclinical investigation of anti-inflammatory agents and IL-1-modulating therapies relevant to human inflammatory, neurologic, and cardiovascular diseases.
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