Rabbit Midkine Recombinant Protein

Catalog Number:
RP1632U
Availability:
In stock
Application:
Cell Culture, Control, ELISA, ELISpot Control, Western Blot Control
100% Homology:
Oryctolagus cuniculus (rabbit)
  • Rabbit Midkine (MK) (catalog RP1632U) is a yeast-derived growth factor supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~13.1 kDa, 120 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to rabbit. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study midkine signaling and cellular processes (including regulation of cell growth, survival, migration, and development); typical experimental uses include cell-culture stimulation and proliferation assays, signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
Amino Acid SequenceKKKDKVKGGP GSECAEWTWG PCTPSSKDCG VGFREGTCGA QTQRIRCRLP CNWKKEFGAD CKYKFDSWGA CDGGTGTKAR QGTLKKARYN AQCQETIRVT KPCTPKTKAK AKAKKGKAKD (120)
EndotoxinNaturally endotoxin-free
FormLyophilized
Storage Conditions-20°C
Molecular Weight13.1 kDa
Purity>98% as visualized by SDS-PAGE analysis.
Expression SystemYeast
FormLyophilized
Country Of OriginUSA
Rabbit Midkine (MK) is a heparin-binding growth factor and cytokine that plays important roles in cell growth, development, and tissue repair in rabbits (Oryctolagus cuniculus). Midkine is highly expressed during embryonic development and can also be induced in adult tissues in response to injury, inflammation, or physiological stress. Rabbit midkine interacts with several cell surface receptors, including receptor-type protein tyrosine phosphatase ζ (PTPRZ1), low-density lipoprotein receptor–related proteins (LRPs), and integrins, activating intracellular signaling pathways such as MAPK, PI3K–AKT, and NF-κB that regulate cell proliferation, migration, survival, and inflammatory responses. Through these mechanisms, midkine contributes to processes including neural development, angiogenesis, immune cell recruitment, and tissue regeneration. In cell culture, recombinant rabbit midkine is commonly used to stimulate cell proliferation and migration and to study growth factor–mediated signaling pathways involved in development, inflammation, and tissue repair. Rabbit midkine is also studied in rabbit animal models of cardiovascular disease, atherosclerosis, and tissue injury, where it helps researchers investigate mechanisms of inflammation, vascular remodeling, and regenerative responses.

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Saint Paul, MN 55114

Phone: 651-646-0089
Fax: 651-646-0095

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