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Swine Midkine Recombinant Protein
- Catalog Number:
- RP1636S
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Sus scrofa (pig)
- Swine Midkine (MK) (catalog RP1636S) is a yeast-derived growth factor supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~13.2 kDa, 121 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to pig. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study midkine signaling and cellular processes (including regulation of cell growth, survival, migration, and development); typical experimental uses include cell-culture stimulation and proliferation assays, signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | KKKDKVKKGG PGSECAEWTW GPCTPSSKDC GVGFREGTCG AQTQRVRCRV PCNWKKEFGA DCKYKFESWG ACDGGTGTKA RQGTLKKARY NAQCQETIRV TKPCTPKTKA KAKAKKGKGK D (121) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 13.2 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Swine Midkine (MK) is a heparin-binding growth factor and cytokine that plays important roles in cell growth, development, and tissue repair in pigs (Sus scrofa). Midkine is highly expressed during embryonic development and can also be induced in adult tissues in response to injury, inflammation, or physiological stress. Swine midkine interacts with several cell surface receptors, including receptor-type protein tyrosine phosphatase ζ (PTPRZ1), low-density lipoprotein receptor-related proteins (LRPs), and integrins, activating intracellular signaling pathways such as MAPK, PI3K-AKT, and NF-κB that regulate cell proliferation, migration, survival, and inflammatory responses. Through these mechanisms, midkine contributes to processes including tissue regeneration, angiogenesis, neural development, and immune cell recruitment. In pigs, midkine is also associated with developmental processes and responses to tissue injury or infection. In cell culture, recombinant swine midkine is commonly used to stimulate cell proliferation and migration and to study growth factor-mediated signaling pathways involved in development, inflammation, and tissue repair. Swine midkine is also studied in veterinary and translational research, as pigs serve as valuable models for human cardiovascular, metabolic, and regenerative biology studies.
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