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Ovine CD40 Ligand (TNFSF5) Recombinant Protein
- Catalog Number:
- RP1638V
- Availability:
- In stock
- Application:
- Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
- 100% Homology:
- Ovis aries (sheep)
- Ovine CD40 Ligand (CD40L, CD154) (catalog RP1638V) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~16.1 kDa, 149 amino acids long (full sequence provided), and >98% pure by SDS-PAGE, with 100% amino-acid homology to sheep. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. It is commonly used to study CD40L signaling and immune responses (including regulation of B-cell activation, antibody production, and T-cell-mediated immunity); typical experimental uses include cell-culture stimulation and activation assays, immune signaling studies, ELISA and neutralization assays, flow-cytometry and Western blot controls, and antibody generation/validation. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use.
| Amino Acid Sequence | MHKGDQEPQI AAHVISEASS KTTSVLQWAP KGYYTLSSNL VTLENGKQLA VKRQGFYYIY TQVTFCSNRE ALNQAPFIAS LCLKSPSGSE RILLRAANTH SSSKPCGQQS IHLGGVFELQ QGASVFVNVT DPSQVSHGTG FTSFGLLKL (149) |
| Endotoxin | Naturally endotoxin-free |
| Storage Conditions | -20°C |
| Molecular Weight | 16.1 kDa |
| Purity | >98% as visualized by SDS-PAGE analysis. |
| Country Of Origin | USA |
- Ovine CD40 Ligand (CD40L), also known as CD154 and encoded by the CD40LG gene, is a type II transmembrane protein that belongs to the tumor necrosis factor (TNF) superfamily, which includes related ligands such as TNF-α, CD27 ligand (CD70), OX40 ligand (CD252), and 4-1BB ligand (CD137L) that regulate immune activation and intercellular signaling. In sheep (Ovis aries), CD40L is primarily expressed on activated CD4⁺ T lymphocytes, and can also be detected on platelets and other activated immune cells following antigen stimulation. CD40L binds to its receptor CD40, which is expressed on B cells, dendritic cells, macrophages, and other antigen-presenting cells, initiating intracellular signaling pathways such as NF-κB and MAPK that promote B-cell activation, proliferation, immunoglobulin class switching, germinal center formation, and development of memory B cells. Through these mechanisms, ovine CD40L plays a critical role in T cell-dependent antibody responses and regulation of adaptive immunity. In sheep, the CD40-CD40L signaling pathway contributes to immune responses against viral, bacterial, and parasitic pathogens, including those associated with respiratory disease, gastrointestinal infections, and parasitic infestations common in small ruminant production systems. Because effective humoral immunity is essential for disease resistance and vaccine-induced protection, ovine CD40L is studied in veterinary immunology, vaccine development, and host-pathogen interaction research, helping to improve strategies for immune protection and disease control in sheep.
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